Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy.
Rotations of a few cross-bridges in muscle by confocal total internal reflection microscopy.
复制标题
通过共焦全内反射显微镜观察肌肉中一些横桥的旋转。
DOI：
10.1016/j.bbamcr.2005.11.011
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Burghardt,TP
中科院分区：
文献类型：
--
作者：
Borejdo,J;Talent,J;Akopova,I;Burghardt,TP
为了测量收缩骨骼肌中几个（~6）肌球蛋白头的循环，通过全内反射照亮肌原纤维并通过共焦孔径进行观察。肌球蛋白头以大约等于 ATP 酶速率的速率旋转，表明整个肌肉的大量 ATP 酶反映了各个头的循环频率。
In order to measure the cycling of a few (∼6) myosin heads in contracting skeletal muscle, myofibrils were illuminated by Total Internal Reflection and observed through a confocal aperture. Myosin heads rotated at a rate approximately equal to the ATPase rate, suggesting that bulk ATPase of a whole muscle reflects the cycle frequency of individual heads.