Effects of cocaine on blood flow and prostaglandin metabolites in rat sciatic nerve.
Effects of cocaine on blood flow and prostaglandin metabolites in rat sciatic nerve.
复制标题
可卡因对大鼠坐骨神经血流和前列腺素代谢的影响。
DOI：
10.1152/ajpheart.1994.266.6.h2515
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Roux,L
中科院分区：
文献类型：
--
作者：
Kalichman,MW;Sanicolas,MT;Jorge,MC;Roux,L
为了更好地了解局麻药减少神经血流量和调节神经血流量的机制，本实验观察了可卡因对麻醉大鼠坐骨神经血流量和血管活性前列腺素的影响。30min后，神经周围注射160mM可卡因[-29.4+/-4.0(SD)激光多普勒血流单位(P<；0.001)]后，神经血流量较基线显著减少，但生理盐水(1.6+/-11.3)不明显。取出这些神经，分别测定血管活性二十烷类前列环素和血栓素A2的稳定代谢物6-酮-前列腺素F1α和血栓素B2。可卡因处理后，两种代谢产物均减少，但血栓素B2/6-酮前列腺素F1α比值较高(6.1+/-3.2 vs.2.4+/-1.1)，且与神经血流量呈负相关(P<；0.01)。在另一项单独的实验中，神经周围注射前列腺素合成抑制剂吲哚美辛(0.5毫克在0.5毫升生理盐水或50%乙醇中)也减少了神经血流量(P<；0.05)。这些结果与可卡因通过影响神经前列腺素代谢产物而抑制神经血流的说法是一致的。
To better understand the mechanisms of local anesthetic-reduced nerve blood flow and nerve blood flow regulation, the effects of cocaine on blood flow and vasoactive prostaglandins were tested in the sciatic nerve of anesthetized rats. After 30 min, nerve blood flow was significantly reduced from baseline by perineural injection of 160 mM cocaine [-29.4 +/- 4.0 (SD) laser-Doppler flow units (P < 0.001)] but not saline (1.6 +/- 11.3). These same nerves were removed and assayed for the stable metabolites 6-ketoprostaglandin F1 alpha and thromboxane B2 of the vasoactive eicosanoids prostacyclin and thromboxane A2, respectively. Both metabolites were reduced, but the ratio of thromboxane B2 to 6-ketoprostaglandin F1 alpha was greater (P < 0.05) in nerves pretreated with cocaine (6.1 +/- 3.2 vs. 2.4 +/- 1.1) and was inversely correlated (P < 0.01) with nerve blood flow. In a separate experiment, perineural injection of the prostaglandin synthesis inhibitor indomethacin (0.5 mg in 0.5 ml of either saline or 50% ethanol) reduced nerve blood flow as well (P < 0.05). These results are consistent with the proposal that cocaine inhibits nerve blood flow by effects on nerve prostaglandin metabolites.