In Vivo Visualization of Vasculature in Adult Zebrafish by Using High-Frequency Ultrafast Ultrasound Imaging
In Vivo Visualization of Vasculature in Adult Zebrafish by Using High-Frequency Ultrafast Ultrasound Imaging
复制标题
DOI：
10.1109/tbme.2018.2878887
复制
发表时间：
2019-06-01
影响因子：
4.6
通讯作者：
Huang, Chih-Chung
中科院分区：
文献类型：
--
作者：
Chang, Chao-Chuan;Chen, Pei-Yu;Huang, Chih-Chung
目的：斑马鱼最近被认为是研究发育生物学和遗传学的理想脊椎动物，特别是在体内模拟肿瘤发生、血管生成和再生。然而，当斑马鱼完全成熟时，它的身体就会失去透明度，从而使传统的光学成像技术难以成像内部解剖和血管系统。声波穿透优于光学方法，因此高频(>；30 MHz)超声(HFUS)是成年斑马鱼成像的替代成像方式，特别是对于超声心动图，然而，使用传统的HFUS显示斑马鱼的周围血管仍然困难。方法：提出了基于超快超声成像的高频微多普勒成像技术用于斑马鱼背侧血管的活体标测。高频MU DI使用40 MHz超声换能器，这是目前可用的最高频率的超快超声成像技术。血流信号的提取使用了不同设置的基于特征的杂波滤波器。实验是在8月龄野生AB系成年斑马鱼身上进行的。结果：斑马鱼背侧的血管，包括节间血管、索状血管、背侧纵向吻合血管和背主动脉，在二维和三维高频超声图像上均清晰可见。结论：高频微泡成像的最大成像深度为4 mm，最小血管直径为36 mm，不需要任何微泡。斑马鱼背侧血管的最大流速范围约为3~4 mm/S。意义：与常规超声多普勒成像相比，高频MU-DI显示了更好的小血管成像。
Objective: Zebrafish has been recently considered an ideal vertebrate for studying developmental biology, genetics, particularly for modeling tumorigenesis, angiogenesis, and regeneration in vivo. However, when a zebrafish matures completely, its body loses transparency, thus making conventional optical imaging techniques difficult for imaging internal anatomy and vasculature. Acoustic wave penetration outperforms optical methods, high-frequency (>30 MHz) ultrasound (HFUS) was consequently an alternative imaging modality for adult zebrafish imaging, particularly for echocardiography However, visualizing peripheral vessels in a zebrafish by using conventional HFUS is still difficult. Methods: In the present study, high-frequency micro-Doppler imaging (HF mu DI) based on ultrafast ultrasound imaging was proposed for zebrafish dorsal vascular mapping in vivo. HF mu DI uses a 40-MHz ultrasound transducer, which is an ultrafast ultrasound imaging technology with the highest frequency available currently. Blood flow signals were extracted using an eigen-based clutter filter with different settings. Experiments were performed on an 8-month-old wild-type AB-line adult zebrafish. Results: Blood vessels, including intersegmental vessels, parachordal vessel, dorsal longitudinal anastomotic vessel, and dorsal aorta, from the dorsal side of the zebrafish were clearly observed in two-dimensional (2-D) and 3-D HF mu DI. Conclusion: The maximum image depth of HF mu DI and the minimal diameter of vessel can be detected were 4 mm and 36 mu m, respectively; they were determined without any use of microbubbles. The maximum flow velocity range was approximately 3-4 mm/s on the dorsal vessels of the adult zebrafish. Significance: Compared with conventional ultrasound Doppler imaging, HF mu DI exhibited superior small vessel imaging.