NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells
NF-κB mediates the antiproliferative and proapoptotic effects of bergamot juice in HepG2 cells
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DOI：
10.1016/j.lfs.2015.12.040
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发表时间：
2016-02-01
期刊：
影响因子：
6.1
通讯作者：
Navarra, Michele
中科院分区：
文献类型：
--
作者：
Ferlazzo, Nadia;Cirmi, Santa;Navarra, Michele
目的：肝细胞癌是世界范围内最常见的癌症之一，其发病率在世界范围内呈上升趋势。许多证据强调，通常在饮食中摄入的天然物质对预防癌症有重要作用。在这项研究中，我们研究了佛手柑汁（BJ）对人肝癌HepG2细胞抗增殖活性的分子机制。主要方法：将HepG2细胞暴露于BJ后，观察细胞增殖、细胞周期进展、细胞凋亡和nf - κ B核易位。关键发现：我们的研究结果表明，BJ以时间和浓度依赖的方式降低了人肝癌HepG2细胞的生长速度，其机制涉及通过内在和外在途径激活凋亡机制。此外，BJ增加了P53和P21蛋白的表达，这可能是导致HepG2细胞周期阻滞在G2期的原因。此外，RI减少NF-kappa B核易位。意义：我们的数据证明了RI具有抑制HepG2细胞生长的能力，揭示了其作用机制，提示了其作为抗癌药物的前景。(C) 2016 Elsevier Inc.版权所有。
Aims: Among cancers, hepatocellular carcinoma is one of the commonest worldwide, and its incidence is increasing around the world. A lot of evidence underlines that natural substances usually consumed in the diet can have an important role in the prevention of cancer. In this study we investigated the molecular mechanisms underlying the antiproliferative activity of Citrus bergamia (bergamot) juice (BJ) in human hepatocellular carcinoma HepG2 cells.Main methods: HepG2 cells were exposed to BJ and then cell proliferation, cell cycle progression, apoptosis and NF-kappa B nuclear translocation were evaluated.Key findings: Here we present results demonstrating that BJ reduced the growth rate of human hepatocellular carcinoma HepG2 cells in a time- and concentration-dependent manner, by a mechanism involving the activation of apoptotic machinery via both intrinsic and extrinsic pathways. Moreover, BJ increased expression of P53 and P21 proteins that may be responsible for the HepG2 cell cycle arrest in G2 phase. In addition, RI reduced NF-kappa B nuclear translocation.Significance: Our data demonstrate the ability of RI in reducing the growth of HepG2 cells, revealing its mechanism of action and suggesting a promising role as anticancer drugs. (C) 2016 Elsevier Inc. All rights reserved.