Killing of Escherichia coli by Myxococcus xanthus in aqueous environments requires exopolysaccharide-dependent physical contact.
Killing of Escherichia coli by Myxococcus xanthus in aqueous environments requires exopolysaccharide-dependent physical contact.
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DOI：
10.1007/s00248-013-0252-x
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发表时间：
2013-10
影响因子：
3.6
通讯作者：
Shi, Wenyuan
中科院分区：
文献类型：
--
作者：
Pan, Hongwei;He, Xuesong;Lux, Renate;Luan, Jia;Shi, Wenyuan
细菌之间基于营养或生态位的竞争是自然环境中普遍存在的现象。这种种间相互作用通常由分泌的可溶性因子和/或直接细胞-细胞接触介导。粘球菌作为土壤中普遍存在的细菌，能够产生多种具有生物活性的次生代谢产物，抑制其他竞争细菌的生长。同时，粘球菌也表现出复杂的捕食行为，这是一种极端的竞争形式，通常通过与猎物细胞的密切接触来刺激，并且在很大程度上取决于固体表面的可用性。粘球菌也可以从水生环境中分离。然而，粘球菌与其他细菌在这种环境中的相互作用的研究仍然有限。在本研究中，利用研究得很好的M。xanthus DK 1622和E.大肠杆菌作为种间相互作用的模型对，我们证明了在水环境中，黄色粘球菌能够以细胞接触依赖的方式杀死大肠杆菌，并且所观察到的接触依赖的杀死需要M. xanthus和E.大肠杆菌细胞。进一步分析表明，M. xanthus在E.大肠杆菌杀伤力，并且与突变体的EPS产量减少密切相关。此外，M. xanthus对不同的细菌具有不同的结合能力，并且细菌菌株不能与M. xanthus可以逃脱杀死，这表明共聚集的特定性质和相互作用细菌的靶向杀死。总之，我们的研究结果表明，EPS介导的，接触依赖性杀死E。coli中的M. xanthus，一种可能促进这种无处不在的细菌在水生环境中生存的策略。
Nutrient or niche-based competition among bacteria is a widespread phenomenon in natural environment. Such inter-species interactions are often mediated by secreted soluble factors and/or direct cell–cell contact. As ubiquitous soil bacteria, Myxococcus species are able to produce a variety of bioactive secondary metabolites to inhibit the growth of other competing bacterial species. Meanwhile, Myxococcus sp. also exhibit sophisticated predatory behavior, an extreme form of competition that is often stimulated by close contact with prey cells and largely depends on the availability of solid surfaces. Myxococcus sp. can also be isolated from aquatic environments. However, studies focusing on the interaction between Myxococcus and other bacteria in such environments are still limited. In this study, using the well-studied M. xanthus DK1622 and E. coli as model interspecies interaction pair, we demonstrated that in a aqueous environment, Myxococcus xanthus was able to kill Escherichia coli in a cell contact-dependent manner, and that the observed contact dependent killing required the formation of co-aggregates between M. xanthus and E. coli cells. Further analysis revealed that exopolysaccharide (EPS), type IV pilus (TFP) and lipopolysaccharide (LPS) mutants of M. xanthus displayed various degrees of attenuation in E. coli killing, and it correlated well with the mutants' reduction in EPS production. In addition, M. xanthus showed differential binding ability to different bacteria, and bacterial strains unable to co-aggregate with M. xanthus can escape the killing, suggesting the specific nature of co-aggregation and the targeted killing of interacting bacteria. In conclusion, our results demonstrated EPS mediated, contact-dependent killing of E. coli by M. xanthus, a strategy that might facilitate the survival of this ubiquitous bacterium in aquatic environments.
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影响因子：
11.3
作者：
Berleman JE;Kirby JR
通讯作者：
Kirby JR
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Morgan, Andrew D.;MacLean, R. Craig;Velicer, Gregory J.
通讯作者：
Velicer, Gregory J.
影响因子：
3.6
作者：
Lu, A;Cho, KY;Shi, WY
通讯作者：
Shi, WY
影响因子：
3.6
作者：
He, Xuesong;Tian, Yan;Shi, Wenyuan
通讯作者：
Shi, Wenyuan