THE SEQUENCE OF 28S RIBOSOMAL-RNA VARIES WITHIN AND BETWEEN HUMAN CELL-LINES
THE SEQUENCE OF 28S RIBOSOMAL-RNA VARIES WITHIN AND BETWEEN HUMAN CELL-LINES
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DOI：
10.1093/nar/21.6.1449
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发表时间：
1993-03-25
影响因子：
14.9
通讯作者：
ANDERSEN, AH
中科院分区：
文献类型：
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作者：
LEFFERS, H;ANDERSEN, AH
28 S核糖体RNA的一级结构构成了一个保守的核心，这是在大多数23 S-样rRNA和扩展片段，发生在序列中的特定位置相似。扩增片段解释了原核生物（古细菌和真细菌）和真核生物rRNA之间的大部分大小差异，并且它们表现出rRNA中独特的序列变异。我们通过对来自9种不同人类细胞系和组织的总共111个V8片段进行测序，研究了其中一个扩增片段V8的序列变异，并发现了35种不同的变体。变异主要发生在两个“热点”，这两个热点在一级序列中相隔170个核苷酸，但在二级结构中是相邻的。V8区段的序列在人细胞系和组织内和之间都不同。真核生物的28 S rRNA的进化的影响进行了讨论，连同可能的功能的扩展段。我们还提出了一个二级结构模型的基础上比较序列分析和化学和酶足迹的V8段。
The primary structure of 28S ribosomal RNA constitutes a conserved core which is similar among most 23S-like rRNAs and expansion segments which occur at specific positions in the sequence. The expansion segments account for most of the size difference between prokaryotic (archaeal and eubacterial) and eukaryotic rRNAs and they exhibit a sequence variation which is unique among rRNAs. We have investigated the sequence variation of one of the expansion segments, V8, by sequencing a total of 111 V8 segments from 9 different human cell lines and tissues and have found 35 different variants. The variation occur mainly at two 'hot spots' which are separated by 170 nucleotides in the primary sequence but are neighbours in the secondary structure. The sequence of V8 segments varies both within and between human cell lines and tissues. The implications for the evolution of the eukaryotic 28S rRNA are discussed together with possible functions of the expansion segments. We also present a secondary structure model for the V8 segment based on comparative sequence analysis and chemical and enzymatic foot printing.