Effects of perfluorooctane sulfonate on action potentials and currents in cultured rat cerebellar Purkinje cells
Effects of perfluorooctane sulfonate on action potentials and currents in cultured rat cerebellar Purkinje cells
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DOI：
10.1016/j.bbrc.2006.10.038
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发表时间：
2006-12-08
影响因子：
3.1
通讯作者：
Ohmori, Harunori
中科院分区：
文献类型：
--
作者：
Harada, Kouji H.;Ishii, Takahiro M.;Ohmori, Harunori
最近，据报告，全氟辛烷磺酸在环境和人类血清中普遍存在，引起了对其健康风险的关注。采用全细胞膜片钳技术研究了全氟辛烷磺酸对培养的大鼠小脑浦肯野细胞动作电位和电流的影响。在电流钳实验中，全氟辛烷磺酸显著降低电流注入过程中的动作电位频率、最大下降速率和动作电位阈值，并在剂量超过30 M时使静息膜电位负移。在电压钳实验中，全氟辛烷磺酸在浓度为30 μ M时使I-Ca、I-Na和I-K的半激活和失活电压向超极化方向移动。在爪蟾卵母细胞中表达的I-HCN 1也受到类似的影响。将全氟辛烷磺酸掺入细胞膜可能会增加表面负电荷密度，从而降低跨膜电位梯度，并导致离子通道激活和失活的超极化偏移。这些研究结果表明，全氟辛烷磺酸可能具有神经毒性。(c)2006爱思唯尔公司All rights reserved.
Recently, PFOS was reported to be ubiquitously detected in the environment, as well as in human serum, raising concerns regarding its health risks. We investigated the effects of PFOS on action potentials and currents in cultured rat cerebellar Purkinje cells using whole-cell patch-clamp recording. In current-clamp experiments, PFOS significantly decreased the action potential frequency during current injection, the maximum rate of fall and the threshold of action potential, and negatively shifted the resting membrane potential at doses over 30 M. In voltage-clamp experiments, PFOS shifted the half-activation and inactivation voltages of I-Ca, I-Na, and I-K toward hyperpolarization at 30 mu M. I-HCN1 expressed in Xenopus oocytes was similarly affected. Incorporation of PFOS into the cell membrane probably increased the surface negative charge density, thereby reducing the transmembrane potential gradient and resulting in hyperpolarizing shifts of both the activation and inactivation of ionic channels. These findings indicate that PFOS may exhibit neurotoxicity. (c) 2006 Elsevier Inc. All rights reserved.