Bacterial production of methylglyoxal: a survival strategy or death by misadventure?
Bacterial production of methylglyoxal: a survival strategy or death by misadventure?
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DOI：
10.1042/bst0311406
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发表时间：
2003-12
影响因子：
3.9
通讯作者：
I. R. Booth;G. P. Ferguson;Sarah Christine Miller;Chan Li;Banuri Gunasekera;S. Kinghorn
中科院分区：
文献类型：
--
作者：
I. R. Booth;G. P. Ferguson;Sarah Christine Miller;Chan Li;Banuri Gunasekera;S. Kinghorn
细菌细胞中 MG（甲基乙二醛）的产生必须与解毒能力和针对这种亲电子试剂的保护能力保持平衡。 MG 的过量产生会导致细胞死亡。革兰氏阴性菌暴露于 MG 后的存活情况最容易被理解。主要的保护机制是 MG 与 GSH 自发反应形成半硫醇缩醛，然后通过乙二醛酶系统解毒，产生 D-乳酸。 KefB 和 KefC 谷胱甘肽门控 K(+) 外排系统与乙二醛酶系统的活性相结合，以响应亲电子试剂的暴露而调节细胞质 pH 值。当新陈代谢发生不平衡时，细菌才会产生 MG。 MG 旁路的运行使细胞能够适应，从而恢复新陈代谢的平衡。 MG 的过量生产是一种适应性策略，如果失败，则会产生致命的后果。在此基础上，人们可能会将重症肌无力引起的死亡定义为“意外死亡”，而不是自杀！
The production of MG (methylglyoxal) in bacterial cells must be maintained in balance with the capacity for detoxification and protection against this electrophile. Excessive production of MG leads to cell death. Survival of exposure to MG is best understood in the Gram-negative bacteria. The major mechanism of protection is the spontaneous reaction of MG with GSH to form hemithiolacetal, followed by detoxification by the glyoxalase system leading to the production of D-lactate. The KefB and KefC glutathione-gated K(+) efflux systems are integrated with the activity of the glyoxalase system to regulate the cytoplasmic pH in response to exposure to electrophiles. Bacteria only produce MG when an imbalance occurs in metabolism. Operation of the MG bypass enables cells to adapt, such that balance is restored to metabolism. Excessive production of MG is an adaptive ploy, which, if it fails, has fatal consequences. On this basis one might define MG-induced loss of life as "death by misadventure" rather than suicide!