Effect of gonadotropin-releasing hormone antagonists on serum follicle-stimulating hormone and luteinizing hormone under conditions of singular follicle-stimulating hormone secretion.
Effect of gonadotropin-releasing hormone antagonists on serum follicle-stimulating hormone and luteinizing hormone under conditions of singular follicle-stimulating hormone secretion.
复制标题
单一促卵泡激素分泌条件下促性腺激素释放激素拮抗剂对血清促卵泡激素和黄体生成素的影响。
DOI：
10.1095/biolreprod32.2.391
复制
发表时间：
1985
影响因子：
3.6
通讯作者：
Vale,WW
中科院分区：
文献类型：
--
作者：
Schwartz,NB;Rivier,C;Rivier,J;Vale,WW
先前的工作表明，在长期去卵巢的大鼠中，促性腺激素释放激素(GnRH)的有效拮抗剂比卵泡刺激素(FSH)更有效地抑制血清黄体生成素(LH)。本研究检测了卵巢摘除后血清FSH的急剧升高，或在发情前期继发高峰期，是否依赖于GnRH。实验A于动情后期0800h摘除大鼠卵巢，于同日1200h或2~5d注射(Ac-dedro-Pro1，PCL-D-PhE2，D-Trp3，6，NaMeLeu7)-GnRH(Antag-I)。Antag-I可完全阻断促黄体生成素反应，但仅部分抑制血清FSH水平。实验B在卵巢切除时注射更高剂量的拮抗剂[(Ac-3-Pro1，Pf-D-PhE2，D-Trp3，6]-GnRH；Antag-II)。该类似物对血清促黄体生成素和促卵泡刺激素的抑制分别为79%和30%。实验C检测了发情前期应用Antag-II对FSH自发二次峰的影响，以及对外源促黄体生成素诱导的二次FSH峰的影响。在发情前期1200h给予Antag-II，可阻止排卵和预计在1830h出现的黄体生成素高峰，以及在1830h和0400h出现的血清FSH升高。实验D在1200h注射Antag-II，1530h摘除卵巢，对照组在0400h时ANTag-II已抑制FSH，而去卵巢大鼠FSH反应强烈。我们得出的结论是，卵巢切除后或促黄体生成激素后发生的单一FSH反应仅对GnRH有微弱的依赖性。
Previous work has indicated that in long-term ovariectomized rats a potent antagonist to gonadotropin-releasing hormone (GnRH) suppressed serum luteinizing hormone (LH) more successfully than follicle-stimulating hormone (FSH). The present studies examined whether the rise in serum FSH which occurs acutely after ovariectomy, or during the proestrous secondary surge, depends on GnRH. In Experiment A, rats were ovariectomized at 0800 h of metestrus and injected with (Ac-dehydro-Pro1, pCl-D-PhE2, D-Trp3,6, NaMeLeu7)-GnRH (Antag-I) at 1200 h of the same day, or 2 or 5 days later. Antag-I blocked the LH response completely, but only partially suppressed serum FSH levels. Experiment B tested a higher dose of a more potent antagonist [( Ac-3-Pro1, pF-D-PhE2, D-Trp3,6]-GnRH; Antag-II) injected at the time of ovariectomy. The analog suppressed serum LH by 79% and FSH by 30%. Experiment C examined the effect of Antag-II on the day of proestrus on the spontaneous secondary surge of FSH, as well as on a secondary FSH surge which can be induced by exogenous LH. Antag-II, given at 1200 h proestrus, blocked ovulation and the LH surge expected at 1830 h, as well as increases in serum FSH which occur at 1830 h and at 0400 h. Exogenous LH triggered a rise in FSH in rats suppressed by Antag-II. In Experiment D proestrous rats were injected with Antag-II at 1200 h and ovariectomized at 1530 h. By 0400 h the antag had suppressed FSH in controls, but in the ovariectomized rats, a vigorous FSH response occurred. We conclude that the singular FSH response which occurs acutely after ovariectomy or following LH is only weakly dependent on GnRH.