Antiphagocytic Effect of Slime from a Mucoid Strain of Pseudomonas aeruginosa
Antiphagocytic Effect of Slime from a Mucoid Strain of Pseudomonas aeruginosa
复制标题
铜绿假单胞菌粘液菌株粘液的抗吞噬作用
作者：
S. Schwarzmann;J. Boring
铜绿假单胞菌的粘液型菌株在琼脂培养基表面生长时产生粘性粘液。已知这些菌株在囊性纤维化患儿的气管支气管树中持续定植。定植可能是由于生物体产生的粘液抑制了吞噬作用。从一个粘液菌株分离的粘液进行了检查，以确定它是否具有体外抗吞噬活性。当在37 ℃无粘液条件下旋转混合物2小时时，铜绿假单胞菌、大肠杆菌和金黄色葡萄球菌的细胞被兔多形核白细胞迅速吞噬。向细菌和白细胞中加入相对少量的粘液抑制吞噬作用，这是通过吞噬杀死生物体来测量的。发现铜绿假单胞菌的抑制作用最完全。
Mucoid strains of Pseudomonas aeruginosa produce a viscid slime when grown on the surface of agar media. These strains are known to colonize persistently the tracheobronchial tree of children with cystic fibrosis. Colonization may result from inhibition of phagocytosis due to slime produced by the organism. Slime separated from one mucoid strain was examined to determine whether it possessed antiphagocytic activity in vitro. Cells of P. aeruginosa, Escherichia coli, and Staphylococcus aureus were rapidly phagocytized by rabbit polymorphonuclear leukocytes when mixtures were rotated for 2 hr at 37 C in the absence of slime. The addition of relatively small amounts of slime to bacteria and leukocytes inhibited phagocytosis as measured by phagocytic killing of the organisms. Inhibition was found to be most complete with P. aeruginosa.