Analysis of immune-related genes during Nora virus infection of Drosophila melanogaster using next generation sequencing.
Analysis of immune-related genes during Nora virus infection of Drosophila melanogaster using next generation sequencing.
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DOI：
10.3934/microbiol.2018.1.123
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发表时间：
2018
影响因子：
4.8
通讯作者：
Carlson KA
中科院分区：
文献类型：
--
作者：
Lopez W;Page AM;Carlson DJ;Ericson BL;Cserhati MF;Guda C;Carlson KA
黑腹果蝇依靠先天免疫系统来调节和对抗病毒感染。这是一个复杂但广泛保守的过程，涉及许多免疫通路和基因相互作用。此外，参与免疫的基因表达随着生物体年龄的增长而受到差异调节。对于表现出慢性感染的病毒来说尤其如此，诺拉病毒就是如此。诺拉病毒是一种持续性的非致病性病毒，在黑腹果蝇中以水平方式复制。参与调节对诺拉病毒感染的免疫反应的基因在很大程度上是未知的。此外，尚未研究感染导致的免疫反应基因的时间响应。在这项研究中，感染诺拉病毒或未感染的黑腹果蝇分别饲养2、10、20和30天。通过下一代测序（NGS）对这些样本的RNA进行分析，并利用PANTHER和DAVID数据库以及与免疫相关基因列表和FlyBase进行比较来评估所得的免疫相关基因。数据表明，随着时间的推移，感染诺拉病毒的黑腹果蝇免疫相关基因表达增加。此外，在第30天，数据表明可能会发生持续性免疫反应，导致特定免疫反应基因的上调。这些结果表明了NGS在确定参与诺拉病毒复制、慢性感染和抗病毒通路的潜在免疫系统基因方面的实用性。
Drosophila melanogaster depends upon the innate immune system to regulate and combat viral infection. This is a complex, yet widely conserved process that involves a number of immune pathways and gene interactions. In addition, expression of genes involved in immunity are differentially regulated as the organism ages. This is particularly true for viruses that demonstrate chronic infection, as is seen with Nora virus. Nora virus is a persistent non-pathogenic virus that replicates in a horizontal manner in D. melanogaster. The genes involved in the regulation of the immune response to Nora virus infection are largely unknown. In addition, the temporal response of immune response genes as a result of infection has not been examined. In this study, D. melanogaster either infected with Nora virus or left uninfected were aged for 2, 10, 20 and 30 days. The RNA from these samples was analyzed by next generation sequencing (NGS) and the resulting immune-related genes evaluated by utilizing both the PANTHER and DAVID databases, as well as comparison to lists of immune related genes and FlyBase. The data demonstrate that Nora virus infected D. melanogaster exhibit an increase in immune related gene expression over time. In addition, at day 30, the data demonstrate that a persistent immune response may occur leading to an upregulation of specific immune response genes. These results demonstrate the utility of NGS in determining the potential immune system genes involved in Nora virus replication, chronic infection and involvement of antiviral pathways.