Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: Restricted array of mutations associated with drug resistance
Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: Restricted array of mutations associated with drug resistance
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DOI：
10.1093/infdis/173.1.196
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发表时间：
1996-01-01
影响因子：
6.4
通讯作者：
vanEmbden, JDA
中科院分区：
文献类型：
--
作者：
Musser, JM;Kapur, V;vanEmbden, JDA
对34株耐药菌株和12株敏感菌株的过氧化氢酶-过氧化物酶基因（katG）和含有异烟肼耐药相关突变的双基因位点（inhA）进行了测序。几乎所有耐药菌都有KatG或inhA上游的氨基酸变化或核苷酸替换，KatG的一个区域编码两个在耐药菌株中经常改变的氨基酸（Ser315和Arg463残基），对来自荷兰的10个易感菌株和51个异烟肼耐药菌株的inhA位点进行了测序，大多数（84%）耐药菌株有KatG或inhA位点突变或缺乏KatG。大约75%的异烟肼耐药菌株在KatG的315或463个氨基酸或inhA上游的核苷酸上发生了替换。所研究的16株牛分枝杆菌和微分枝杆菌在KatG中均含有Leu463而非Arg463，这与Leu463是结核分枝杆菌祖先条件的假设一致。
The catalase-peroxidase gene (katG) and a two-gene locus (inhA) containing mutations associated with resistance to isoniazid in Mycobacterium tuberculosis were sequenced in 34 resistant and 12 susceptible strains. Virtually all resistant organisms had amino acid changes in KatG or nucleotide substitutions upstream of inhA, A region of katG encoding two amino acids frequently altered in resistant strains (residues Ser315 and Arg463) and the inhA locus were sequenced in 10 susceptible and 51 isoniazid-resistant isolates from the Netherlands, Most (84%) of the resistant isolates had mutations in katG or the inhA locus or lacked katG, Together, similar to 75% of isoniazid-resistant isolates had replacements at amino acids 315 or 463 in KatG or nucleotide substitutions upstream of inhA. All 16 strains of Mycobacterium bovis and Mycobacterium microti studied had Leu463 rather than Arg463 in KatG, an observation consistent with the hypothesis that Leu463 is the ancestral condition in M. tuberculosis.