Extracellular ATP released by copper stress could act as diffusible signal in alleviating the copper stress-induced cell death
Extracellular ATP released by copper stress could act as diffusible signal in alleviating the copper stress-induced cell death
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铜应激释放的细胞外 ATP 可以作为缓解铜应激诱导的细胞死亡的扩散信号
DOI：
10.1007/s00709-018-1309-y
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发表时间：
2019-03-01
期刊：
影响因子：
2.9
通讯作者：
Feng, Han-Qing
中科院分区：
文献类型：
--
作者：
Jia, Ling-Yun;Bai, Jing-Yue;Feng, Han-Qing
本工作利用烟草细胞悬浮液和小麦幼苗，研究了铜胁迫释放的eATP(细胞外ATP)在缓解铜胁迫诱导的细胞死亡中可能起到的扩散信号作用。将半透膜固定在塑料盒的中间，将塑料盒分为两个相等的隔室(分别为A和B)。这种半透膜可以防止细胞与细胞(或幼苗与幼苗)之间的直接接触和大分子[如ATPase(三磷酸腺苷)]在这两个隔室之间的扩散。将CuCl2直接胁迫的细胞悬浮液放置在A室，与未经处理的细胞悬液在B室孵育，显著提高了这两个室细胞悬浮液的细胞死亡水平和eATP含量。相反，与不添加ATPase相比，在CuCl2直接胁迫的细胞悬浮液中加入ATPase降低了这两个隔室的eATP水平，但进一步增加了B隔室的细胞死亡水平。用小麦幼苗代替烟草细胞悬浮液也得到了类似的结果。这些观察表明，当来自A室的铜胁迫诱导B室的植物细胞死亡时，从A室转移的ATP可能起到缓解这种细胞死亡的作用。因此，铜胁迫释放的eATP可能在缓解铜胁迫诱导的细胞死亡中起扩散信号的作用。
In the present work, by using tobacco cell suspension and wheat seedlings, we studied that eATP (extracellular ATP) released by copper (Cu) stress could act as diffusible signal in alleviating the Cu stress-induced cell death. A semipermeable membrane was fixed in the middle of a plastic box to divide the box into two equal compartments (A and B, respectively). This semipermeable membrane can prevent direct cell-to-cell (or seedling-to-seedling) contact and the diffusion of the macromolecules [such as ATPase (adenosine 5′-triphosphatase)] between these two compartments. The cell suspension directly stressed with CuCl2was placed in compartment A and was incubated with the untreated cell suspension in compartment B. Such treatment significantly increased the levels of cell death and eATP content of the cell suspension in these two compartments. In contrast, addition of ATPase into the cell suspension directly stressed with CuCl2decreased the eATP level in these two compartments but further increased the level of cell death in compartment B, compared to no addition of ATPase. Similar results were obtained when tobacco cell suspension was replaced by wheat seedlings. These observations indicate that when Cu stress from compartment A induced the plant cell death in compartment B, ATP transferred from compartment A could play a role in alleviating this cell death. Thus, it is suggested that eATP released by copper stress could act as diffusible signal in alleviating the Cu stress-induced cell death.