Variable cytocompatibility of six cell lines with photoinitiators used for polymerizing hydrogels and cell encapsulation
Variable cytocompatibility of six cell lines with photoinitiators used for polymerizing hydrogels and cell encapsulation
复制标题
DOI：
10.1016/j.biomaterials.2004.04.024
复制
发表时间：
2005-04-01
期刊：
影响因子：
14
通讯作者：
Elisseeff, JH
中科院分区：
文献类型：
--
作者：
Williams, CG;Malik, AN;Elisseeff, JH
用于生物医学和组织工程应用的生物相容性光聚合聚合物的开发具有降低设计用于修复或增强组织的生物材料植入物的侵入性和成本的潜力。然而，需要更多关于这些系统中使用的化合物和引发剂的细胞毒性的信息。目前的研究评估了三种紫外线敏感的光引发剂对六种不同细胞群的细胞毒性，这些细胞群用于工程化许多组织。光引发剂2-羟基-1-[4-（羟基乙氧基）苯基]-2-甲基-1-丙酮（Irgacure 2959）在广泛的哺乳动物细胞类型和种属中引起最小毒性（细胞死亡）。还证明了不同的细胞类型对相同浓度的相同光引发剂具有可变的响应。虽然细胞系中的固有差异可能导致可变的细胞毒性，但观察到细胞增殖速率（群体倍增时间）与光引发剂的细胞毒性增加之间存在相关性。分裂更快的细胞系对光引发剂诱导的细胞死亡更敏感。总之，光引发剂Irgacure 2959在一系列哺乳动物物种的许多细胞类型中耐受良好。可以优化细胞光包封策略以通过操纵增殖速率来改善细胞存活。(C)2004爱思唯尔有限公司保留所有权利。
The development of biocompatible photopolymerizing polymers for biomedical and tissue engineering applications has the potential to reduce the invasiveness and cost of biomaterial implants designed to repair or augment tissues. However, more information is needed about the cellular toxicity of the compounds and initiators used in these systems. The current study evaluates the cellular toxicity of three ultraviolet sensitive photoinitiators on six different cell populations that are used for engineering numerous tissues. The photoinitiator 2-hydroxy-1-[4-(hydroxyethoxy)phenyl]-2-methyl-1-propanone (Irgacure 2959) caused minimal toxicity (cell death) over a broad range of mammalian cell types and species. It was also demonstrated that different cell types have variable responses to identical concentrations of the same photoinitiator. While inherent differences in the cell lines may contribute to the variable cytotoxicity, a correlation between cellular proliferation rate (population doubling time) and increased cytotoxicity of the photoinitiator was observed. Cell lines that divided more quickly were more sensitive to photoinitiator-induced cell death. In summary, the photoinitiator Irgacure 2959 is well tolerated by many cell types over a range of mammalian species. Cell photoencapsulation strategies may be optimized to improve cell survival by manipulating proliferation rate. (C) 2004 Elsevier Ltd. All rights reserved.