2-Aminopurine inhibits RNA and protein synthesis and reduces catecholamine desensitization in C6-2B rat glioma cells.
2-Aminopurine inhibits RNA and protein synthesis and reduces catecholamine desensitization in C6-2B rat glioma cells.
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2-Aminopurine 抑制 C6-2B 大鼠神经胶质瘤细胞中的 RNA 和蛋白质合成并减少儿茶酚胺脱敏。
DOI：
10.1016/0014-5793(92)80415-d
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
Brooker,G
中科院分区：
文献类型：
--
作者：
DeBernardi,MA;Brooker,G
我们以前提出，细胞内环磷酸腺苷积累诱导一个推定的，迅速翻转蛋白抑制进一步激素激活腺苷酸环化酶。在本研究中，2-氨基嘌呤，据报道，选择性阻断c-fosgene表达，被用来测试的假设，c-fos蛋白可能参与脱敏过程。事实上，在2-氨基嘌呤处理的C6 - 2B大鼠胶质瘤细胞中观察到对儿茶酚胺的异源脱敏减少。然而，我们发现2-氨基嘌呤以浓度依赖性方式抑制C6 - 2B中的总细胞RNA和蛋白质合成。HeLa、Swiss 3 T3和BALB/e细胞。在2-氨基嘌呤处理的细胞中，mRNA合成也显著减少。这些意想不到的发现，虽然支持我们的假设，即蛋白质合成敏感步骤在难治性的发展中，引起了对2-氨基嘌呤抑制c-fos诱导作用的特异性的关注，因此任何细胞过程，包括脱敏，这可能是由c-fosgene表达调节的。
We previously proposed that intracellular cyclic AMP accumulation induces a putative, rapidly turning over protein inhibitory to further hormone activation of adenylate cyclase. In the present study, 2‐aminopurine, which has been reported to selectively block c‐fosgene expression, was used to test the hypothesis that c‐fos protein might be involved in the desensitization process. Indeed, a reduction in heterologous desensitization to catecholamines was observed in 2‐aminopurine‐treated C6‐2B rat glioma cells. However, we found 2‐aminopurine to inhibit, in a concentration‐dependent manner, total cellular RNA and protein synthesis in C6‐2B. HeLa, Swiss 3T3 and BALB/e cells. mRNA synthesis was also markedly reduced in 2‐aminopurine‐treated cells. These unexpected findings, while supporting our hypothesis of a protein synthesis‐sensitive step in the development of refractoriness, raise concern about the specificity of action of 2‐aminopurine to inhibit c‐fosinduction and thus any cellular process, including desensitization, which might be regulated by c‐fosgene expression.