Surrobodies with functional tails.
Surrobodies with functional tails.
复制标题
带有功能性尾巴的替代体。
DOI：
10.1016/j.jmb.2010.01.036
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发表时间：
2010
影响因子：
5.6
通讯作者：
R. Lerner
中科院分区：
文献类型：
--
作者：
Li Xu;A. Estellés;R. Briante;A. Kurtzman;C. Hannum;A. Kashyap;Lawrence Horowitz;M. Horowitz;R. R. Bhatt;R. Lerner
替代体2是一种基于前B细胞受体（pre-BCR）的独特类型的结合蛋白。前BCR在抗体库发育期间瞬时表达。与由相同的重链和轻链对组成的异源四聚体典型抗体不同，前BCR是由相同的重链对组成的异源六聚体复合物，所述重链对各自与两亚基替代轻链（SLC）配对。SLC在两个SLC组分上均含有非免疫球蛋白样肽延伸。这种安排提供了独特的机会，蛋白质工程的功能衍生这些nonimmunoglobulin样尾巴。在这里，我们报告了与这些尾部的重组融合，其具有完全活性的细胞因子或具有单链可变片段（scFv）结构域，以产生具有独特功能的替代体或在靶向结合方面是双特异性的替代体。
Surrobodies2are a unique type of binding protein based on the pre-B-cell receptor (pre-BCR). The pre-BCR is transiently expressed during development of the antibody repertoire. Unlike heterotetrameric canonical antibodies that are composed of identical pairs of heavy and light chains, the pre-BCR is a heterohexameric complex composed of identical pairs of heavy chains that are each paired with a two-subunit surrogate light chain (SLC). The SLC contains nonimmunoglobulin-like peptide extensions on each of the two SLC components. This arrangement provides unique opportunities for protein engineering by functional derivatization of these nonimmunoglobulin-like tails. Here we report recombinant fusions to these tails with either a fully active cytokine or with single-chain variable fragment (scFv) domains to generate Surrobodies with unique functions or Surrobodies that are bispecific with respect to targeted binding.
影响因子：
11.2
作者：
Xiaochuan Xu;P. Clarke;G. Szalai;J. Shively;L. Williams;Y. Shyr;Ergang Shi;F. Primus
通讯作者：
Xiaochuan Xu;P. Clarke;G. Szalai;J. Shively;L. Williams;Y. Shyr;Ergang Shi;F. Primus
DOI：
10.1073/pnas.91.20.9626
发表时间：
1994-09
影响因子：
11.1
作者：
Helen Sabzevari;S. D. Gillies;Barbara M. Mueller;J. Pancook;Ralph A. Reisfeld
通讯作者：
Helen Sabzevari;S. D. Gillies;Barbara M. Mueller;J. Pancook;Ralph A. Reisfeld