A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis
A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis
复制标题
DOI：
10.1016/j.febslet.2004.11.070
复制
发表时间：
2005-01-03
期刊：
影响因子：
3.5
通讯作者：
Mukai, K
中科院分区：
文献类型：
--
作者：
Kuroda, M;Oikawa, K;Mukai, K
2，3，7，8-四氯二苯并二恶英（TCDD）是一种内分泌干扰物，具有多种毒性作用。我们已经从小鼠胚胎干细胞中分离出了一个小鼠同源的hWAPL基因，称为小鼠WAPL（mWAPL），作为TCDD的目标，通过cDNA代表性差异分析。在AhR（-/-）小鼠胚胎成纤维细胞中，在0.1 μ m TCDD下观察到mWAPL表达的统计学显著增加。有趣的是，在1 mum TCDD时，AhR（+/+）细胞中WAPL mRNA水平降低，但AhR（-/-）细胞中进一步增加。hWAPL和mWAPL仅在正常组织样本中的睾丸中高表达，并且我们观察到mWAPL定位在睾丸染色体的联会复合体中。此外，小鼠睾丸mWAPL mRNA的表达减少后，单次腹腔注射TCDD。因此，哺乳动物WAPL如hWAPL和mWAPL可能参与精子发生，并可能是介导TCDD生殖毒性的靶基因。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine disruptor that produces a variety of toxic effects. We have isolated a mouse homolog of the hWAPL gene, termed mouse WAPL (mWAPL), as a target of TCDD by cDNA representational difference analysis from mouse embryonic stern cells. A statistically significant increase in mWAPL expression was observed at 0.1 mum TCDD in AhR(-/-) mouse embryonic fibroblast cells. Interestingly at 1 mum TCDD, in WAPL mRNA levels decreased in AhR(+/+) cells, but further increased in AhR(-/-) cells. hWAPL and mWAPL were highly expressed only in testes among normal tissue samples, and we observed mWAPL localization in the synaptonemal complex of testicular chromosomes. In addition, mouse testes decreased the expression of mWAPL mRNA after a single intraperitoneal injection of TCDD. Thus, mammalian WAPL such as hWAPL and mWAPL may be involved in spermatogenesis and be target genes mediating the reproductive toxicity induced by TCDD. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.