Overexpression of a soybean O-acetylserine (thiol) lyase-encoding gene GmOASTL4 in tobacco increases cysteine levels and enhances tolerance to cadmium stress
Overexpression of a soybean O-acetylserine (thiol) lyase-encoding gene GmOASTL4 in tobacco increases cysteine levels and enhances tolerance to cadmium stress
复制标题
烟草中大豆 O-乙酰丝氨酸（硫醇）裂解酶编码基因 GmOASTL4 的过度表达可增加半胱氨酸水平并增强对镉胁迫的耐受性
DOI：
10.1007/s10529-009-0178-z
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发表时间：
2010-04-01
影响因子：
2.7
通讯作者：
Yu, Deyue
中科院分区：
文献类型：
--
作者：
Ning, Huixia;Zhang, Chunhong;Yu, Deyue
O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, OASTL activity and cysteine levels increased significantly in transgenic plants. Cadmium accumulation and the activity of both superoxide dismutase and catalase enzymes were enhanced in transformants. These results demonstrate that overexpression of GmOASTL4 in tobacco can enhance cysteine levels and increase tolerance to cadmium stress.
