Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma
Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma
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DOI：
10.1016/j.wneu.2017.12.166
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发表时间：
2018-03-01
影响因子：
2
通讯作者：
Honmou, Osamu
中科院分区：
文献类型：
--
作者：
Ookawa, Satoshi;Wanibuchi, Masahiko;Honmou, Osamu
背景：SERPINA 1在保护组织免受蛋白水解机制的影响中发挥抗炎作用。免疫组化分析显示SERPINA 1在胶质瘤中呈阳性，然而，SERPINA 1的作用，包括与预后的关系，一直不确定。近年来，数字聚合酶链反应（digital polymerase chain reaction，PCR）已成为检测福尔马林固定石蜡包埋（formalin-fixed paraffin-embedded，FFPE）组织中信使RNA表达的一种超灵敏的方法。目的：本研究应用数字聚合酶链反应（digital PCR）定量检测高级别胶质瘤（high-grade glioma，HGG）中SERPINA 1的表达，并分析其与预后的关系。来自HGG患者的29份FFPE手术样本（7份为世界卫生组织[WHO] III级，22份为WHO IV级）和人胶质母细胞瘤细胞系U87和U118用于分析。结果：免疫组化染色结果显示SERPINA 1在胶质瘤组织和多形性胶质母细胞瘤细胞系中均有表达。数字PCR分析显示，SERPINA 1在III级和IV级HGG患者的组织中的表达率分别为14.3%和63.6%（P = 0.035）。SERPINA 1低表达组的中位生存期为38.8个月，高于高表达组的15.3个月（P = 0.030）。SERPINA 1的高表达提示HGG预后不良。
BACKGROUND: SERPINA1 plays an anti-inflammatory role in protecting tissues from proteolytic mechanisms. SERPINA1 is positive in gliomas by immunohistochemical analysis; however, the role of SERPINA1, including the relationship with prognosis, has been uncertain. In recent years, digital polymerase chain reaction (PCR) has provided ultra-sensitive assessment of messenger RNA expression from formalin-fixed paraffin-embedded (FFPE) tissues.OBJECTIVE: In this study, we quantitatively determined the expression of SERPINA1 in high-grade gliomas (HGGs) using digital PCR, and we analyzed its relationship with prognosis.METHODS: Twenty-nine FFPE surgical samples from patients with HGGs (7 of World Health Organization [WHO] grade III and 22 of WHO grade IV), and human glioblastoma cell lines, U87 and U118, were used for analysis. A qualitative assessment using immunostaining and quantitative assessment using digital PCR were performed to assess the expression of SERPINA1.RESULTS: The expression of SERPINA1 was demonstrated in glioma tissues and glioblastoma multiforme cell lines by immunostaining. Digital PCR analysis showed that SERPINA1 was expressed in 14.3% and 63.6% of the tissues from patients with grade III and grade IV HGG, respectively (P = 0.035). The median overall survival of 38.8 months in the low SERPINA1 expression group was longer than that of 15.3 months in the high expression group (P = 0.030).CONCLUSIONS: The frequency and the amount of SERPINA1 expression were higher in grade IV than in grade III HGGs. The high expression of SERPINA1 indicates a poor prognosis of HGGs.