Targeted Delivery of Sucrose-Coated Nanocarriers with Chemical Cargoes to the Plant Vasculature Enhances Long-Distance Translocation.
Targeted Delivery of Sucrose-Coated Nanocarriers with Chemical Cargoes to the Plant Vasculature Enhances Long-Distance Translocation.
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DOI：
10.1002/smll.202304588
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发表时间：
2023-10
期刊：
影响因子：
13.3
通讯作者：
Su-Ji Jeon;Yilin Zhang;Chris Castillo;Valeria Nava;Kurt Ristroph;Benjamin Therrien;Leticia Meza;Gregory V Lowry;J. P. Giraldo
中科院分区：
文献类型：
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作者：
Su-Ji Jeon;Yilin Zhang;Chris Castillo;Valeria Nava;Kurt Ristroph;Benjamin Therrien;Leticia Meza;Gregory V Lowry;J. P. Giraldo
目前施用农用化学品的做法效率低下，只有一小部分在植物中达到预定目标。纳米载体的表面被蔗糖功能化，使其能够快速有效地通过叶片输送到植物韧皮部，韧皮部是一种维管组织，在整个植物中运输糖、信号分子和农用化学品。蔗糖分子与韧皮部细胞上糖膜转运蛋白的化学亲和性增强了蔗糖包被量子点（sucQD）和具有β-环糊精分子筐的生物相容性碳点（如β- cd）的吸收，这些碳点可以携带多种农用化学品。QD和CD荧光发射特性可以通过共聚焦和荧光显微镜检测和监测小麦叶片脉管系统的快速易位（<40 min）。这些β- cds使化学物质进入叶维管组织的能力增加了一倍以上。电感耦合等离子体质谱（ICP-MS）分析表明，经修饰后的QDs进入韧皮部并转运到根中的比例比未修饰的高6.8倍以上。纳米颗粒的蔗糖包被方法能够前所未有地靶向递送到根，约70%的韧皮部负载纳米颗粒递送到根。利用植物生物识别分子介导的递送为将含有农用化学品的纳米载体引导到植物脉管系统和整个植物提供了一种有效的方法。
Current practices for delivering agrochemicals are inefficient, with only a fraction reaching the intended targets in plants. The surfaces of nanocarriers are functionalized with sucrose, enabling rapid and efficient foliar delivery into the plant phloem, a vascular tissue that transports sugars, signaling molecules, and agrochemicals through the whole plant. The chemical affinity of sucrose molecules to sugar membrane transporters on the phloem cells enhances the uptake of sucrose-coated quantum dots (sucQD) and biocompatible carbon dots with β-cyclodextrin molecular baskets (suc-β-CD) that can carry a wide range of agrochemicals. The QD and CD fluorescence emission properties allowed detection and monitoring of rapid translocation (<40 min) in the vasculature of wheat leaves by confocal and epifluorescence microscopy. The suc-β-CDs more than doubled the delivery of chemical cargoes into the leaf vascular tissue. Inductively coupled plasma mass spectrometry (ICP-MS) analysis showed that the fraction of sucQDs loaded into the phloem and transported to roots is over 6.8 times higher than unmodified QDs. The sucrose coating of nanoparticles approach enables unprecedented targeted delivery to roots with ≈70% of phloem-loaded nanoparticles delivered to roots. The use of plant biorecognition molecules mediated delivery provides an efficient approach for guiding nanocarriers containing agrochemicals to the plant vasculature and whole plants.