Lack of production of (p)ppGpp in Halobacterium volcanii under conditions that are effective in the eubacteria
Lack of production of (p)ppGpp in Halobacterium volcanii under conditions that are effective in the eubacteria
复制标题
在对真细菌有效的条件下，火山盐杆菌中缺乏 (p)ppGpp 的产生
作者：
G. Scoarughi;C. Cimmino;P. Donini
严格的盐细菌菌株 Haloferax volcanii 经历了一系列不同于氨基酸饥饿的生理条件，已知这些条件会通过 relA 独立 (spoT) 途径在真细菌中导致鸟苷多磷酸 [(p)pp Gpp] 的产生。使用的条件是温度升高、氰化物处理和完全饥饿。在这些条件下均未观察到可检测水平的 (p)ppGpp。这一结果与我们之前的发现（p）ppGpp 合成在氨基酸饥饿下不会发生相结合，得出这样的结论：在盐细菌中，生长速率控制和严格性可能是由在不存在 ppGpp 的情况下运行的机制控制的。在指数生长过程中，观察到低水平的磷酸化化合物，其电泳迁移率与 (p)ppGpp 相似但不相同。这些化合物的细胞内浓度在生长稳定期以及使用的所有处理中显着增加。这些化合物被鉴定为短链多磷酸盐，与在酿酒酵母中类似条件下发现的化合物相同。
The stringent halobacterial strain Haloferax volcanii was subjected to a set of physiological conditions different from amino acid starvation that are known to cause production of guanosine polyphosphates [(p)pp Gpp] in eubacteria via the relA-independent (spoT) pathway. The conditions used were temperature upshift, treatment with cyanide, and total starvation. Under none of these conditions were detectable levels of (p)ppGpp observed. This result, in conjunction with our previous finding that (p)ppGpp synthesis does not occur under amino acid starvation, leads to the conclusion that in halobacteria both growth rate control and stringency are probably governed by mechanisms that operate in the absence of ppGpp. During exponential growth, a low level of phosphorylated compounds with electrophoretic mobilities similar, but not identical, to that of (p)ppGpp were observed. The intracellular concentration of these compounds increased considerably during the stationary phase of growth and with all of the treatments used. The compounds were identified as short-chain polyphosphates identical to those found under similar conditions in Saccharomyces cerevisiae.
DOI：
10.1073/pnas.87.14.5533
发表时间：
1990-07-01
影响因子：
11.1
作者：
GAAL, T;GOURSE, RL
通讯作者：
GOURSE, RL