Effects of staphylococcal infection and aseptic inflammation on bone mass and biomechanical properties in a rabbit model
Effects of staphylococcal infection and aseptic inflammation on bone mass and biomechanical properties in a rabbit model
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葡萄球菌感染和无菌炎症对兔模型骨量和生物力学特性的影响
DOI：
10.1016/j.jot.2019.11.006
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发表时间：
2020-03-01
影响因子：
6.6
通讯作者：
Yue, Bing
中科院分区：
文献类型：
--
作者：
Li, Hui;Zhang, Shutao;Yue, Bing
背景/目的：骨科植入物是旨在缓解疼痛和改善活动度的重要器械。葡萄球菌感染和无菌性松动是导致植入物失效的两种与炎性骨质溶解相关的常见事件。骨量和生物力学特性是可能影响翻修手术后患者结局的重要指标。然而，细菌感染的动力学及其对骨量和生物力学特性的影响仍不清楚。因此，在这项研究中，我们开发了兔无菌性炎症和葡萄球菌感染模型，以确定凝固酶阳性和凝固酶阴性细菌感染，以及无菌性炎症，对骨的质量和生物力学性能的影响。方法：60只新西兰白色兔随机分为6组，每组10只。在每组家兔的髓腔内注射磷酸盐缓冲盐水（100 μ L）、钛（Ti）磨损颗粒（300 μ g/100 μ L）、低浓度表皮葡萄球菌（105/100 μ L）、高浓度S.表皮葡萄球菌（108/100 μ L）、低浓度金黄色葡萄球菌（105/100 μ L）和高浓度S.金黄色葡萄球菌（108/100 μ L）。在手术后4周和8周，处死兔子，通过组织病理学、显微计算机断层扫描和纳米压痕测试分析手术侧的胫骨。组织学分析表明，高浓度葡萄球菌感染，特别是凝固酶阳性葡萄球菌感染，比低浓度的细菌感染和钛磨损颗粒更有害。同时，显微计算机断层扫描和纳米压痕测试表明，高浓度的S。结论：高浓度凝固酶阳性葡萄球菌引起的炎性骨溶解与低骨量和生物力学性能受损显著相关。本文的翻译潜力：在翻修手术前对骨质量和生物力学性能进行全面评估是必要的，尤其是当S。涉及金黄色葡萄球菌感染。此外，更好地了解这两个参数可能有助于制定合理的治疗方案，降低翻修手术后不良事件的风险。
Background/objective: Orthopaedic implants are important devices aimed at relieving pain and improving mobility. Staphylococcal infection and aseptic loosening are two common events associated with inflammatory osteolysis that lead to implant failures. Bone mass and biomechanical properties are important indicators that could influence patient outcomes after revision surgery. However, the dynamics of bacterial infections and their influence on bone mass and biomechanical properties remain unclear. Hence, in this study, we developed rabbit aseptic inflammation and staphylococcal infection models to determine the effects of coagulase-positive and coagulase-negative bacterial infection, as well as aseptic inflammation, on the mass and biomechanical properties of the bone.Methods: Sixty New Zealand white rabbits were randomly assigned to 6 groups, and each group had 10 rabbits. The medullary cavities in rabbits of each group were injected with phosphate-buffered saline (100 mu L), titanium (Ti)-wear particles (300 mu g/100 mu L), a low concentration of Staphylococcus epidermidis (105/100 mu L), a high concentration of S. epidermidis (108/100 mu L), a low concentration of Staphylococcus aureus (105/100 mu L), and a high concentration of S. aureus (108/100 mu L), respectively. At four and eight weeks after surgery, the rabbits were sacrificed, and the tibias on the surgical side were analysed via histopathology, microcomputed tomography, and nanoindentation testing.Results: Histopathological analysis demonstrated that inflammatory responses and bacterial loads caused by high concentrations of staphylococcal infections, particularly coagulase-positive staphylococci, are more detrimental than low concentrations of bacterial infection and Ti-wear particles. Meanwhile, microcomputed tomography and nanoindentation testing showed that high concentrations of S. aureus caused the highest loss in bone mass and most biomechanical function impairment in rabbits experiencing aseptic inflammation and staphylococcal infections.Conclusions: Inflammatory osteolysis caused by a high concentration of coagulase-positive staphylococci is significantly associated with low bone mass and impaired biomechanical properties.The translational potential of this article: It is necessary to obtain an overall assessment of the bone mass and biomechanical properties before revision surgery, especially when S. aureus infection is involved. In addition, a better understanding of these two parameters might help develop a reasonable treatment regimen and reduce the risk of adverse events after revision surgery.