Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR.
Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR.
复制标题
对真菌、植物、原生生物和动物的总基因组 DNA 进行微波小量制备，用于 PCR。
作者：
Goodwin Dc;Steven B. Lee
描述了从各种真核生物中分离总DNA的快速程序。这种方法使用普通的微波炉来改变细胞壁和细胞膜。这一过程非常迅速，只需15分钟即可完成。该方法已被用于从爬行动物、植物、黏菌和其他原生生物中分离DNA，这些DNA已被用于成功扩增这些生物的核糖体基因。该程序是一种节省时间和成本效益的过程，有助于消除其他DNA分离程序中可能发现的几种污染源。
A rapid procedure for the isolation of total DNA from various eukaryotic organisms is described. This method uses a common microwave oven for the alteration of cellular walls and membranes. The procedure is rapid and can be completed in as little as 15 minutes. The method has been used to isolate DNA from reptiles, plants, slime molds and other protists, and this DNA has been used for successful amplification of ribosomal genes from these organisms. The procedure is a time-saving and cost-effective process that helps to eliminate several sources of contamination that can be found in other DNA isolation procedures.