Simultaneous analysis of the oxidation of solvent-extracted and cold-pressed green coffee oil during accelerated storage using 1H NMR and 13C NMR spectroscopy
Simultaneous analysis of the oxidation of solvent-extracted and cold-pressed green coffee oil during accelerated storage using 1H NMR and 13C NMR spectroscopy
复制标题
使用 1H NMR 和 13C NMR 光谱同时分析溶剂提取和冷榨绿咖啡油在加速储存过程中的氧化情况
DOI：
10.1016/j.foodres.2023.112470
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发表时间：
2023
影响因子：
8.1
通讯作者：
Yuzhou Long
中科院分区：
文献类型：
--
作者：
Wenjiang Dong;Qidi Hong;Jinhuan Cheng;Hongyan He;Yanan Li;Rongsuo Hu;Yuzhou Long
绿咖啡油（GCO）是从生咖啡豆中提取的，以其抗氧化和抗癌特性而闻名，在化妆品和其他消费品中的应用越来越多。然而，GCO脂肪酸组分在储存过程中的脂质氧化可能对人体健康有害，对GCO化学组分氧化的演变仍有必要了解。本研究采用质子核磁共振（1H和13c NMR）波谱法研究了溶剂萃取和冷压GCO在加速储存条件下的氧化状态。结果表明，随着氧化时间的延长，氧化产物的信号强度逐渐增强，而不饱和脂肪酸的信号逐渐减弱。除了在主成分分析的二维平面上有少量的重叠外，根据其性质对5种不同类型的GCO提取物进行聚类。偏最小二乘最小分析结果表明，1h NMR氧化产物（δ= 7.8 ~ 10.3 ppm）、不饱和脂肪酸（δ= 5.28 ~ 5.42 ppm）和亚油酸（δ= 2.70 ~ 2.85 ppm）可以作为GCO氧化水平的特征指标。加速贮藏36 d时，不饱和脂肪酸、亚油酸和亚麻酸酰基的动力学曲线均符合指数方程，GCO系数较高。研究结果表明，现有的核磁共振系统是一种快速、简便、方便的氧化过程监测和质量控制工具。
Green coffee oil (GCO) extracted from green coffee beans, is known for its antioxidant and anticancer properties, and has been increasingly utilised in cosmetic and other consumer products. However, lipid oxidation of GCO fatty acid components during storage may be harmful to human health, and there remains a need to understand the evolution of GCO chemical component oxidation. In this study, proton nuclear magnetic resonance (1H and13C NMR) spectroscopy was used to investigate the oxidation status of solvent-extracted and cold-pressed GCO under accelerated storage conditions. Results show that the signal intensity of oxidation products gradually increased with increasing oxidation time, while unsaturated fatty acid signals gradually weakened. Five different types of GCO extracts were clustered according to their properties, except for minor overlapping in the two-dimensional plane of the principal component analysis. Partial least squares-least analysis results demonstrate that oxidation products (δ= 7.8–10.3 ppm), unsaturated fatty acids (δ= 5.28–5.42 ppm), and linoleic acid (δ= 2.70–2.85 ppm) in1H NMR can be used as characteristic indicators of GCO oxidation levels. Furthermore, the kinetics curves of unsaturated fatty acids, linoleic, and linolenic acyl groups all fit an exponential equation with high coefficients of GCO for 36 days under accelerated storage conditions. Our results show that the current NMR system is a fast, easy-operated and convenient tool for the oxidation process monitoring and quality control of GCO.