MULTI-FLORET SPIKELET1, Which Encodes an AP2/ERF Protein, Determines Spikelet Meristem Fate and Sterile Lemma Identity in Rice
MULTI-FLORET SPIKELET1, Which Encodes an AP2/ERF Protein, Determines Spikelet Meristem Fate and Sterile Lemma Identity in Rice
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DOI：
10.1104/pp.113.216044
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发表时间：
2013-06-01
期刊：
影响因子：
7.4
通讯作者：
He, Guanghua
中科院分区：
文献类型：
--
作者：
Ren, Deyong;Li, Yunfeng;He, Guanghua
小穗是一种独特的草的花序结构。控制小穗发育的分子机制尚不清楚。在本研究中，我们鉴定了一个水稻小穗突变体，多花小穗1(Mfs1)，它表现出小穗分生组织向花分生组织转化的延迟，从而导致额外的壳状器官和拉长的小穗轴。此外，在mfs1中，不育的外稃被同源地转换为不发育的颖片，内颖体退化。这些结果表明，多花SPIKELET1(MFS1)基因在小穗分生组织决定和花器官鉴定中起着重要的调节作用。MFS1属于APETALA2/乙烯反应因子(AP2/ERF)家族中一个未知的功能分支。MFS1-绿色荧光蛋白融合蛋白定位于细胞核。MFS1信使RNA在多种组织中表达，特别是在小穗和花分生组织中。此外，我们的发现表明，MFS1正向调节长不育外颖和不确定的类似SPIKELET1(IDS1)的基因--多余的苞叶和OsIDS1的表达。
The spikelet is a unique inflorescence structure of grass. The molecular mechanism that controls the development of the spikelet remains unclear. In this study, we identified a rice (Oryza sativa) spikelet mutant, multi-floret spikelet1 (mfs1), that showed delayed transformation of spikelet meristems to floral meristems, which resulted in an extra hull-like organ and an elongated rachilla. In addition, the sterile lemma was homeotically converted to the rudimentary glume and the body of the palea was degenerated in mfs1. These results suggest that the MULTI-FLORET SPIKELET1 (MFS1) gene plays an important role in the regulation of spikelet meristem determinacy and floral organ identity. MFS1 belongs to an unknown function clade in the APETALA2/ethylene-responsive factor (AP2/ERF) family. The MFS1-green fluorescent protein fusion protein is localized in the nucleus. MFS1 messenger RNA is expressed in various tissues, especially in the spikelet and floral meristems. Furthermore, our findings suggest that MFS1 positively regulates the expression of LONG STERILE LEMMA and the INDETERMINATE SPIKELET1 (IDS1)-like genes SUPERNUMERARY BRACT and OsIDS1.