Dinucleotide repeat polymorphism at the D21S172 locus.
Dinucleotide repeat polymorphism at the D21S172 locus.
复制标题
D21S172 基因座的二核苷酸重复多态性。
DOI：
10.1093/nar/19.5.1169-a
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发表时间：
1991
影响因子：
14.9
通讯作者：
M. Litt
中科院分区：
文献类型：
--
作者：
V. Sharma;L. Allen;R. Magenis;M. Litt
阿尔--077 A2=.103 A3=。217 A4=.013 A5=.013 A6=.051 A7=.525PIC=0.62染色体定位与孟德尔遗传：荧光原位杂交定位粘粒ICRFc102D0922至lp31。2.对2个CEPH家系进行连锁分析，发现14个信息性减数分裂位点与D1S22(定位于PTER-p22(1))无重组，最高LOD评分为4。50at theta=0。所有病例均观察到孟德尔遗传。扩增条件：总体积为25tl，包括：基因组DNA 25 ng，每个引物10pmole，1.5 mM氯化镁，200，IM dNTPs，50 mM KCI，10 mM Tris-Cl，pH 8.3，0.6单位Taq聚合酶(BRL)和0.01%明胶。扩增周期为35个循环，94℃变性30秒，46℃退火45秒，72℃延伸1.5分钟。V.Sharma是俄勒冈州医学研究基金会的奖学金获得者。我们感谢H.Lehrach提供了COSMID ICRFc1O2DO922。参考文献：1)Bruns，Gap和Sherman，SL(1989)Cytogenet。细胞基因组。51，67-90。
Al-. 077 A2=. 103 A3=. 217 A4=. 013 A5=. 013 A6=. 051 A7=. 525 PIC= 0.62 Chromosomal Localization and Mendelian Inheritance: Fluorescence in situ hybridizationlocalized cosmid ICRFc102D0922 to lp31. 2. Linkage analysis in 2 CEPH families showed no recombination in 14 informative meioses with D1S22 (locWalized to pter-p22 (1)), giving a maximum LOD score of4. 50 at theta= 0. Mendelian inheritance was observed in all cases. PCR Conditions: We carry out PCR in a total volume of 25 tl containing: 25 ng genomic DNA, 10 pmole of each primer, 1.5 mM MgCl2, 200, IM dNTPs, 50 mM KCI, 10 mM tris-Cl, pH 8.3, 0.6 units Taq polymerase (BRL) and 0.01% gelatin. Amplification is for 35 cycles with denaturation at 94 C for 30 seconds, annealing at 46 C for 45 seconds and extension at 72 C for 1.5 minutes.Acknowledgements: Thiswork was supported by grant HG00022 from the National Institutes of Health. V. Sharma is the recipient ofa fellowship from the Medical Research Foundation of Oregon. We thank H. Lehrach for providing cosmid ICRFc1O2DO922. Reference: 1) Bruns, GAP and Sherman, SL (1989) Cytogenet. Cell Genet. 51, 67-90.