Immune responses and protection obtained with rotavirus VP6 DNA vaccines given by intramuscular injection.
Immune responses and protection obtained with rotavirus VP6 DNA vaccines given by intramuscular injection.
复制标题
通过肌肉注射轮状病毒 VP6 DNA 疫苗获得的免疫反应和保护。
DOI：
10.1016/s0264-410x(00)00543-0
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发表时间：
2001
期刊：
影响因子：
5.5
通讯作者：
Herrmann,JE
中科院分区：
文献类型：
--
作者：
Yang,K;Wang,S;Chang,KO;Lu,S;Saif,LJ;Greenberg,HB;Brinker,JP;Herrmann,JE
肌内（i.m.）注射鼠源性VP 6 DNA疫苗后，BALB/c小鼠血清中产生了较高滴度的轮状病毒特异性IgG和伊加抗体。基于血清IgG 2a与IgG 1抗体的比率产生Th 1样抗体应答。轮状病毒特异性血清伊加，但不是粪便伊加检测轮状病毒攻击前小鼠。通过粪便中轮状病毒抗原脱落的减少来测量，实现了对轮状病毒攻击的部分保护。用牛轮状病毒VP 6 DNA疫苗对鼠轮状病毒攻击发现类似水平的保护，表明可以通过用VP 6 DNA疫苗免疫获得异源保护。我们没有直接测试细胞毒性T淋巴细胞（CTL）的活性，但在体内消耗的CD 8 +T细胞免疫小鼠与鼠VP 6 DNA疫苗没有显着改变病毒脱落的持续时间或获得的保护模式。这一发现表明，CD 8 +CTL活性不是我们通过i.m.用VP 6 DNA疫苗免疫小鼠。
Intramuscular (i.m.) injection of murine VP6 DNA vaccines raised high titers of rotavirus-specific serum IgG and IgA antibodies in BALB/c mice. A Th1-like antibody response was generated based on the ratio of serum IgG2a to IgG1 antibodies. Rotavirus-specific serum IgA but not fecal IgA was detected in mice prior to rotavirus challenge. Partial protection against rotavirus challenge was achieved as measured by reduction of rotavirus antigen shedding in feces. A similar level of protection was found with a bovine rotavirus VP6 DNA vaccine against a murine rotavirus challenge, suggesting that heterologous protection can be obtained by immunizing with VP6 DNA vaccines. We did not directly test for cytotoxic T lymphocyte (CTL) activity, but in vivo depletion of CD8+T cells in mice immunized with a murine VP6 DNA vaccine did not significantly change the duration of virus shedding or the pattern of protection obtained. This finding suggested that CD8+CTL activity was not essential for the partial protection we obtained by i.m. immunization of mice with VP6 DNA vaccines.