Role of nitric oxide in adenosine-induced vasodilation in humans
Role of nitric oxide in adenosine-induced vasodilation in humans
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DOI：
10.1161/01.hyp.31.5.1061
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发表时间：
1998-05-01
期刊：
影响因子：
8.3
通讯作者：
Biaggioni, I
中科院分区：
文献类型：
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作者：
Costa, F;Biaggioni, I
血管舒张是腺苷最突出的作用之一，也是最早被认识到的作用之一，但其作用机制尚不完全清楚。特别是，关于内皮因子的潜在作用存在相互矛盾的信息。本研究的目的是探讨一氧化氮在腺苷血管扩张作用中的作用，在臂内输注生理盐水或一氧化氮合酶抑制剂n - g -单甲基- l-精氨酸（L-NMMA） （12.5 mg/min）时，用静脉阻塞容积描图评估前臂腺苷输注（125 μ g/min）时的血流反应，臂内输注乙酰胆碱（50 μ g/min）和硝普苷（3 μ g/min）作为阳性和阴性对照。分别。选择这些剂量是为了产生相当水平的血管舒张。在另一项研究中，第二次生理盐水输注代替L-NMMA，以排除与时间相关的影响。正如预期的那样，L-NMMA预处理降低了乙酰胆碱诱导的血管舒张；50 μ g/min乙酰胆碱在生理盐水和L-NMMA输注时分别使前臂血流量增加150+/-43%和51+/-12% (P
Vasodilation is one of the most prominent effects of adenosine and one of the first to be recognized, but its mechanism of action is not completely understood. In particular, there is conflicting information about the potential contribution of endothelial factors. The purpose of this study was to explore the role of nitric oxide in the vasodilatory effect of adenosine, Forearm blood flow responses to intrabrachial adenosine infusion (125 mu g/min) were assessed with venous occlusion plethysmography during intrabrachial infusion of saline or the nitric oxide synthase inhibitor N-G-monomethyl-L-arginine (L-NMMA) (12.5 mg/min), Intrabrachial infusions of acetylcholine (50 mu g/min) and nitroprusside (3 mu g/min) were used as a positive and negative control, respectively. These doses were chosen to produce comparable levels of vasodilation. In a separate study, a second saline infusion was administered instead of L-NMMA to rule out time-related effects. As expected, pretreatment with L-NMMA reduced acetylcholine-induced vasodilation; 50 mu g/min acetylcholine increased forearm blood flow by 150+/-43% and 51+/-12% during saline and L-NMMA infusion, respectively (P