Pathological lesions in the central nervous system and peripheral tissues of ddY mice with street rabies virus (1088 strain).
Pathological lesions in the central nervous system and peripheral tissues of ddY mice with street rabies virus (1088 strain).
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DOI：
10.1292/jvms.17-0028
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发表时间：
2017-06-10
期刊：
影响因子：
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通讯作者：
Park CH
中科院分区：
文献类型：
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作者：
Kimitsuki K;Yamada K;Shiwa N;Inoue S;Nishizono A;Park CH
在动物模型中对狂犬病病毒致病机制的研究大多采用固定的狂犬病病毒，而使用街头狂犬病病毒的研究结果并不一致。因此，为了阐明街头狂犬病病毒1088株在小鼠体内的致病机制，将106个病灶形成单位接种于ddy小鼠(6周龄，雌性)的右后肢。接种后3d，后肢肌肉出现轻度炎症。5DPI时，右侧腰骶段脊髓背根神经节内神经节细胞染色变性。脊髓背角和背根神经节的轴突变性和炎性细胞随着感染的进展而增加。术后7DPI出现右后肢瘫痪，进展为四肢瘫痪。而脊髓前角和根纤维未见病理改变。病毒抗原在3d时首先在右侧后肢肌肉中检出，然后在5d时在右侧腰骶背根神经节、脊髓背角、左侧红核、延髓和大脑皮质(M1区)检测到病毒抗原。这些结果表明，1088病毒在感染早期主要通过传入纤维向腰骶髓上升，并通过下行脊髓束向大脑皮层(M1区)移动。此外，我们得出结论，感染1088菌株的小鼠的脊髓感觉束发生了显著的病理变化；这种选择性的敏感性导致了这种疾病的临床特征。
Most studies on rabies virus pathogenesis in animal models have employed fixed rabies viruses, and the results of those employing street rabies viruses have been inconsistent. Therefore, to clarify the pathogenesis of street rabies virus (1088 strain) in mice, 106 focus forming units were inoculated into the right hindlimb of ddY mice (6 weeks, female). At 3 days postinoculation (DPI), mild inflammation was observed in the hindlimb muscle. At 5 DPI, ganglion cells in the right lumbosacral spinal dorsal root ganglia showed chromatolysis. Axonal degeneration and inflammatory cells increased with infection progress in the spinal dorsal horn and dorsal root ganglia. Right hindlimb paralysis was observed from 7 DPI, which progressed to quadriparalysis. However, no pathological changes were observed in the ventral horn and root fibers of the spinal cord. Viral antigen was first detected in the right hindlimb muscle at 3 DPI, followed by the right lumbosacral dorsal root ganglia, dorsal horn of spinal cord, left red nuclei, medulla oblongata and cerebral cortex (M1 area) at 5 DPI. These results suggested that the 1088 virus ascended the lumbosacral spinal cord via mainly afferent fibers at early stage of infection and moved to cerebral cortex (M1 area) using descending spinal tract. Additionally, we concluded that significant pathological changes in mice infected with 1088 strain occur in the sensory tract of the spinal cord; this selective susceptibility results in clinical features of the disease.