Hydrogel-based drug delivery systems: Comparison of drug diffusivity and release kinetics
Hydrogel-based drug delivery systems: Comparison of drug diffusivity and release kinetics
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DOI：
10.1016/j.jconrel.2009.10.030
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发表时间：
2010-03-03
影响因子：
10.8
通讯作者：
Goepferich, Achim
中科院分区：
文献类型：
--
作者：
Brandl, Ferdinand;Kastner, Fritz;Goepferich, Achim
水凝胶作为大分子受控释放的基质被广泛研究。为了评估嵌入分子的流动性，这些药物递送系统通常通过释放研究来表征。然而，这些实验非常耗时，而且可靠性往往很差。在这项研究中，凝胶是通过聚乙二醇（PEG）的逐步聚合制备的，并负载异硫氰酸荧光素（FITC）标记的葡聚糖。机械测试和膨胀研究可以预测预期的 FITC-葡聚糖扩散率。通过光漂白后荧光恢复 (FRAP) 和脉冲场梯度 NMR 光谱测量掺入的 FITC-葡聚糖的平移扩散系数 (D)。由于 D 的测定值与释放研究中获得的值非常吻合，因此建议机械测试、FRAP 和脉冲场梯度 NMR 光谱作为释放实验的替代方案。所应用的方法相互补充并正确地代表了测试样品之间的相对差异。因此，测量 D 可用于快速评估新开发的药物输送系统的潜力。 (C) 2009 Elsevier B.V. 保留所有权利。
Hydrogels are extensively studied as matrices for the controlled release of macromolecules. To evaluate the mobility of embedded molecules, these drug delivery systems are usually characterized by release studies. However, these experiments are time-consuming and their reliability is often poor. In this study, gels were prepared by step-growth polymerization of poly(ethylene glycol) (PEG) and loaded with fluoresceine isothiocyanate (FITC) labeled dextrans. Mechanical testing and swelling studies allowed prediction of the expected FITC-dextran diffusivity. The translational diffusion coefficients (D) of the incorporated FITC-dextrans were measured by fluorescence recovery after photobleaching (FRAP) and pulsed field gradient NMR spectroscopy. Because the determined values of D agreed well with those obtained from release studies, mechanical testing, FRAP, and pulsed field gradient NMR spectroscopy are proposed as alternatives to release experiments. The applied methods complemented each other and represented the relative differences between the tested samples correctly. Measuring D can therefore be used to rapidly evaluate the potential of newly developed drug delivery systems. (C) 2009 Elsevier B.V. All rights reserved.