Oral vaccination with liposome-encapsulated recombinant fusion peptide of urease B epitope and cholera toxin B subunit affords prophylactic and therapeutic effects against H. pylori infection in BALB/c mice.
Oral vaccination with liposome-encapsulated recombinant fusion peptide of urease B epitope and cholera toxin B subunit affords prophylactic and therapeutic effects against H. pylori infection in BALB/c mice.
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DOI：
10.1016/j.vaccine.2007.08.034
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发表时间：
2007-11
期刊：
影响因子：
5.5
通讯作者：
Wenfeng Zhao;Wutong Wu;Xudong Xu
中科院分区：
文献类型：
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作者：
Wenfeng Zhao;Wutong Wu;Xudong Xu
在大肠杆菌中表达了霍乱毒素B亚基与幽门螺杆菌尿素酶B亚基表位的新型融合肽CtUBE。利用该融合肽制备了抗H. pylori感染并在BALB/c小鼠中进行评价。根据尿素酶试验、小鼠胃内可培养菌落的定量和胃炎的组织学鉴定结果，经胃内接种该CtUBE脂质体疫苗后，小鼠具有显著的保护作用，其增加了预防性和治疗性接种方案中特异性抗尿素酶血清IgG和粘膜伊加的含量水平。结果表明，融合肽CtUBE具有免疫原性，可作为口服抗H.幽门感染
A new fusion peptide CtUBE of cholera toxin B subunit and Helicobacter pylori urease B subunit epitope was expressed in Escherichia coli. With this fusion peptide, an oral liposome vaccine against H. pylori infection was prepared and evaluated in BALB/c mice. Based on the results of urease tests, quantitation of culturable bacteria colonies in mice stomachs and histological identification of gastritis, the mice were protected significantly after intragastric vaccination with this CtUBE liposome vaccine, which increased the content levels of specific anti-urease serum IgG and mucosal IgA for both prophylactic and therapeutic vaccination protocols. These results showed that the fusion peptide CtUBE retained immunogenicity and could be used as antigen in the development of an oral vaccine against H. pylori infection.