EFFECTS OF SEX AND ANDROGEN TREATMENT ON DENDRITIC DIMENSIONS OF NEURONS IN THE SEXUALLY DIMORPHIC PREOPTIC ANTERIOR HYPOTHALAMIC AREA OF MALE AND FEMALE FERRETS
EFFECTS OF SEX AND ANDROGEN TREATMENT ON DENDRITIC DIMENSIONS OF NEURONS IN THE SEXUALLY DIMORPHIC PREOPTIC ANTERIOR HYPOTHALAMIC AREA OF MALE AND FEMALE FERRETS
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DOI：
10.1002/cne.903230410
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发表时间：
1992-09-22
影响因子：
2.5
通讯作者：
BAUM, MJ
中科院分区：
文献类型：
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作者：
CHERRY, JA;TOBET, SA;BAUM, MJ
以前在Nissl染色组织中发现了白鼬视前区/下丘脑前区（POA/AH）背侧边缘的一组性二态细胞。在这项研究中，高尔基体染色的组织进行了检查，以1）确定是否存在性别差异，在树突的神经元从这个区域的尺寸，和2）以评估成年雄激素治疗的影响，在两种性别的雪貂树突形态。成年雪貂的大脑给予每日注射丙酸睾酮（5毫克/公斤体重）或油媒剂5周后，性腺切除术浸渍Golgi-Cox程序。在120 μ m切片后，从12只雪貂的性二态性POA/AH中选出78个多极神经元，并借助计算机辅助神经元追踪系统进行三维重建。大的性别差异，观察到在体面积和大多数方面的树突形态，包括总长度，分支的数量，和总树突表面积。雄激素似乎也加重了两种性别的树突状分支，但这种影响比性别效应弱，男性比女性更明显，并限于较少的变量。成年雄激素治疗对男性总树突表面积和四级树突百分比以及女性平均树突厚度的影响最具统计学意义。这些数据表明，强烈的性别差异存在于树突状结构的神经元在POA/AH，并建议在成年期性腺类固醇水平的改变可能会促进突触重塑的大脑区域参与控制性二态行为。
A sexually dimorphic group of cells at the dorsal border of the preoptic/anterior hypothalamic area (POA/AH) of ferrets has been previously identified in Nissl-stained tissue. In this study, Golgi-stained tissue was examined in order 1) to determine whether sex differences exist in dendritic dimensions of neurons from this region, and 2) to assess the effects of adult androgen treatment on dendritic morphology in ferrets of both sexes. Brains from adult ferrets given daily injections of testosterone propionate (5 mg/kg body weight) or oil vehicle for 5 weeks after gonadectomy were impregnated by Golgi-Cox procedures. After sectioning at 120-mu-m, 78 multipolar neurons were selected from the sexually dimorphic POA/AH of 12 ferrets and reconstructed in three dimensions with the aid of a computer-assisted neuron tracing system. Large sex differences were observed in somal area and most aspects of dendritic morphology, including total length, number of branches, and total dendritic surface area. Androgen also appeared to accentuate dendritic arborization in both sexes, but this effect was weaker than the sex effect, more apparent in males than females, and restricted to fewer variables. The most statistically significant effects of adult androgen treatment in males were found for total dendritic surface area and percentage of fourth order dendrites, and in females, average dendritic thickness. These data show that strong sex differences exist in dendritic structure of neurons in the POA/AH, and suggest that alterations in levels of gonadal steroids in adulthood may promote synaptic remodeling in a region of the brain involved in the control of sexually dimorphic behaviors.