Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission
Centriolin anchoring of exocyst and SNARE complexes at the midbody is required for secretory-vesicle-mediated abscission
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DOI：
10.1016/j.cell.2005.07.027
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发表时间：
2005-10-07
期刊：
影响因子：
64.5
通讯作者：
Doxsey, SJ
中科院分区：
文献类型：
--
作者：
Gromley, A;Yeaman, C;Doxsey, SJ
细胞质分裂的最后一步，称为脱落，需要两个未来的子细胞之间的膜连接的分解。我们之前的研究表明，在细胞分裂过程中，卷曲卷曲的蛋白中心蛋白定位于中间体，并且是分离所必需的。本研究表明，中心蛋白与靶向囊泡的囊泡复合物和囊泡融合的SNARE复合物的蛋白相互作用。这些复合物需要向心脂来定位到一个独特的中间环结构，并且任何复合物的破坏都会抑制脱落。囊胞破裂诱导含有v- snare的囊泡在中体环聚集。在对照细胞中，这些v-SNARE囊泡与gfp标记的分泌多肽共定位。囊泡从一个准子细胞不对称地向中体环移动；GFP信号迅速消失，提示膜融合；随后细胞在囊泡传递/融合的地方分裂。我们提出，中心蛋白锚定了囊泡靶向和融合所需的蛋白质复合物，并将膜-囊泡融合与分离结合起来。
The terminal step in cytokinesis, called abscission, requires resolution of the membrane connection between two prospective daughter cells. Our previous studies demonstrated that the coiled-coil protein centriolin localized to the midbody during cytokinesis and was required for abscission. Here we show that centriolin interacts with proteins of vesicle-targeting exocyst complexes and vesicle-fusion SNARE complexes. These complexes require centriolin for localization to a unique midbody-ring structure, and disruption of either complex inhibits abscission. Exocyst disruption induces accumulation of v-SNARE-containing vesicles at the midbody ring. In control cells, these v-SNARE vesicles colocalize with a GFP-tagged secreted polypeptide. The vesicles move to the midbody ring asymmetrically from one prospective daughter cell; the GFP signal is rapidly lost, suggesting membrane fusion; and subsequently the cell cleaves at the site of vesicle delivery/fusion. We propose that centriolin anchors protein complexes required for vesicle targeting and fusion and integrates membrane-vesicle fusion with abscission.