Phylogeny and genomic organization of the TIR and non-TIR NBS-LRR resistance gene family in Medicago truncatula
Phylogeny and genomic organization of the TIR and non-TIR NBS-LRR resistance gene family in Medicago truncatula
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DOI：
10.1094/mpmi.2002.15.6.529
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发表时间：
2002-06-01
影响因子：
3.5
通讯作者：
Cook, DR
中科院分区：
文献类型：
--
作者：
Zhu, HY;Cannon, SB;Cook, DR
从模式豆科植物M中获得了与富含亮氨酸重复序列（LRR）抗性基因的核苷酸结合位点（NBS）结构域同源的序列。通过几种方法。系统发育分析将这些序列分为TIR（Toll和白细胞介素-1受体）和非TIR NBS亚家族，并进一步将它们细分为每个亚家族内的几个明确定义的分支。比较M。truncatula的NBS序列与来自几种密切相关的豆科植物（包括三叶草族（Trifoleae）、豆科（Viceae）和菜豆族（Phaseoleae）的成员）的NBS序列的比较揭示，大多数分支包含来自多个豆科物种的序列。此外，来自密切相关的三叶草族和豆科族内的物种的序列（例如，苜蓿属（Medicago）和豌豆属（Pisum spp.）倾向于同系的并且不同于菜豆族物种的序列（例如，大豆和菜豆）。这些结果表明，NBS-LRR家族内的主要分支的起源早于这些蝶形豆辐射，而这些序列的持续多样化反映了这个豆类亚科内的物种形成。详细的遗传和物理定位的TIR和非TIR NBS序列在M。truncatula表明，大多数NBS序列被组织成簇，并且很少（如果有的话）簇包含TIR和非TIR序列两者。然而，发现的例子，物理集群包含TIR或非TIR亚科内的不同系统发育分支的序列。比较作图揭示了几个同线的抗性基因位点。蒺藜和大豆之间以及M.蒺藜和豌豆
Sequences homologous to the nucleotide binding site (NBS) domain of NBS-leucine-rich repeat (LRR) resistance genes were retrieved from the model legume M. truncatula through several methods. Phylogenetic analysis classified these sequences into TIR (toll and interleukin-1 receptor) and non-TIR NBS subfamilies and further subclassified them into several well-defined clades within each subfamily. Comparison of M. truncatula NBS sequences with those from several closely related legumes, including members of the tribes Trifoleae, Viceae, and Phaseoleae, reveals that most clades contain sequences from multiple legume species. Moreover, sequences from species within the closely related Trifoleae and Viceae tribes (e.g., Medicago and Pisum spp.) tended to be cophyletic and distinct from sequences of Phaseoleae species (e.g., soybean and bean). These results suggest that the origin of major clades within the NBS-LRR family predate radiation of these Papilionoid legumes, while continued diversification of these sequences mirrors speciation within this legume subfamily. Detailed genetic and physical mapping of both TIR and non-TIR NBS sequences in M. truncatula reveals that most NBS sequences are organized into clusters, and few, if any, clusters contain both TIR and non-TIR sequences. Examples were found, however, of physical clusters that contain sequences from distinct phylogenetic clades within the TIR or non-TIR subfamilies. Comparative mapping reveals several blocks of resistance gene loci that are syntenic between M. truncatula and soybean and between M. truncatula and pea.