Identification of the MN antigen as a diagnostic biomarker of cervical intraepithelial squamous and glandular neoplasia and cervical carcinomas.
Identification of the MN antigen as a diagnostic biomarker of cervical intraepithelial squamous and glandular neoplasia and cervical carcinomas.
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发表时间：
1994-09
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通讯作者：
Shu-Yuan Liao;Cheryl Brewer;Jan;Zavada;J. Pastorek;S. Pastoreková;A. Manetta;Michael L. Berman;P. Disaia;J. Eric;Stanbridgell
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作者：
Shu-Yuan Liao;Cheryl Brewer;Jan;Zavada;J. Pastorek;S. Pastoreková;A. Manetta;Michael L. Berman;P. Disaia;J. Eric;Stanbridgell
一个新的肿瘤相关抗原，MN，已被确定其表达与HeLa X成纤维细胞体细胞杂种的致瘤表型相关。由于HeLa是一种来源于宫颈癌的细胞系，我们研究了MN表达在宫颈瘤变中的诊断效用。结果发现，正常宫颈组织不表达抗原，或者在储备细胞弱染色的偶尔局灶性区域表达抗原。相反，在宫颈上皮内瘤变、原位腺癌和鳞状细胞癌和腺癌中观察到显著的免疫反应性（石蜡包埋切片的免疫过氧化物酶染色）。观察到不同的免疫反应性模式，其特征为弥漫性、弥漫性/局灶性或局灶性。超过90%的异型增生或恶性组织显示免疫反应性。一个有趣的观察结果是，与宫颈上皮内瘤变或原位腺癌相关的正常宫颈组织在储备细胞和/或正常柱状细胞中显示出与发育异常组织所表现出的强度水平接近的染色模式。这些结果表明，MN抗原表达作为宫颈肿瘤的生物标志物具有潜在的效用。
A new tumor-associated antigen, MN, has been identified whose expression correlates with the tumorigenic phenotype of HeLa x fibroblast somatic cell hybrids. Because HeLa is a cell line derived from a cervical carcinoma, we investigated the diagnostic utility of MN expression in cervical neoplasia. It was found that normal cervical tissue does not express the antigen, or does so in occasional focal areas of weakly staining reserve cells. In contrast, significant immunoreactivity (immunoperoxidase staining of paraffin-embedded sections) was observed in cervical intraepithelial neoplasias, adenocarcinoma in situ, and frank carcinomas, both squamous cell and adenocarcinoma. Varying patterns of immunoreactivity were observed and were characterized as diffuse, diffuse/focal, or focal. Greater than 90% of dysplastic or malignant tissues showed immunoreactivity. An interesting observation was that normal cervical tissue associated with cervical intraepithelial neoplasias, or adenocarcinoma in situ showed a staining pattern in the reserve cells and/or normal columnar cells that approximated the level of intensity exhibited by the dysplastic tissue. These results indicate that MN antigen expression has potential utility as a biomarker of cervical neoplasms.