Therapeutic hypothermia attenuates acute lung injury in paraquat intoxication in rats
Therapeutic hypothermia attenuates acute lung injury in paraquat intoxication in rats
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DOI：
10.1016/j.resuscitation.2010.11.028
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发表时间：
2011-04-01
期刊：
影响因子：
6.5
通讯作者：
Alam, Hasan B.
中科院分区：
文献类型：
--
作者：
Jo, You Hwan;Kim, Kyuseok;Alam, Hasan B.
研究目的：百草枯中毒可引起急性肺损伤，并有大量死亡报告。百草枯毒性作用的机制是氧化损伤和炎症反应。已知治疗性低温具有抗氧化和抗炎作用。本研究旨在评估治疗性低温对百草枯中毒的影响。方法：雄性sd - dawley大鼠腹腔注射50 mg/kg百草枯，给药1 h后分为常温组（36 ~ 38℃）和低温组（30 ~ 32℃）。低温组先进行2h的低温，再进行2h的复温。在生存研究中，观察百草枯给药后24小时的死亡率。在第二个实验中，在百草枯给药后6小时采集肺组织和血浆。结果：低温组12 h生存率显著高于常温组（100% vs. 50%, p < 0.05）， 24 h生存率无显著差异。低温组急性肺损伤评分低于常温组（p < 0.05）。HT组大鼠肺组织丙二醛含量、血浆白细胞介素-6和亚硝酸盐/硝酸盐浓度显著低于NT组（p < 0.05）。结论：低温治疗延缓了百草枯中毒的早期死亡率，减轻了急性肺损伤。2010爱思唯尔爱尔兰有限公司版权所有。
Aim of the study: Paraquat intoxication induces acute lung injury and numerous fatalities have been reported. The mechanism of toxic effect of paraquat is oxidative injury and inflammation. Therapeutic hypothermia has been known to have antioxidant and anti-inflammatory effects. This study was designed to evaluate the effect of therapeutic hypothermia on paraquat intoxication.Methods: Male Sprague-Dawley rats were given 50 mg/kg of paraquat intraperitoneally and divided into the normothermia (36-38 degrees C) group and the hypothermia (30-32 degrees C) group after 1 h of paraquat administration. The hypothermia group underwent 2 h of hypothermia followed by 2 h of rewarming. In the survival study, mortality was observed for 24 h after paraquat administration. An in the second experiment, lung tissues and plasma were harvested at 6 h after paraquat administration.Results: The 12 h survival rate was significantly higher in the hypothermia group than in the normothermia group (100% vs. 50%, p < 0.05), but survival rates for 24 h were not different. Acute lung injury score was lower in the hypothermia group than in the normothermia group (p < 0.05). Thmalondialdehyde contents of lung tissues, plasma interleukin-6 and nitrite/nitrate concentrations were significantly decreased in the HT group compared to the NT group (p < 0.05).Conclusion: Therapeutic hypothermia delayed early mortality and attenuated acute lung injury in paraquat intoxication. (C) 2010 Elsevier Ireland Ltd. All rights reserved.