THE N-END RULE IS MEDIATED BY THE UBC2(RAD6) UBIQUITIN-CONJUGATING ENZYME
THE N-END RULE IS MEDIATED BY THE UBC2(RAD6) UBIQUITIN-CONJUGATING ENZYME
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DOI：
10.1073/pnas.88.16.7351
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发表时间：
1991-08-01
影响因子：
11.1
通讯作者：
VARSHAVSKY, A
中科院分区：
文献类型：
--
作者：
DOHMEN, RJ;MADURA, K;VARSHAVSKY, A
N-末端规则将蛋白质的体内半衰期与其氨基末端残基的身份联系起来。从哺乳动物到细菌，所有被检测的生物体都有不同版本的N端规则。我们发现，UBC 2（RAD 6），至少有七个泛素结合酶在酵母酿酒酵母，是必不可少的多泛素化和降解的N-末端规则基板。我们还表明，UBC 2是物理上与UBR 1，识别组件的N端规则途径。这些结果表明，UBC 2的一些功能，其中包括DNA修复，诱导诱变，孢子形成，并调节反转录转座，介导的蛋白质降解通过N-末端规则途径。
The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, from mammals to bacteria. We show that UBC2(RAD6), one of at least seven ubiquitin-conjugating enzymes in the yeast Saccharomyces cerevisiae, is essential for multiubiquitination and degradation of the N-end rule substrates. We also show that UBC2 is physically associated with UBR1, the recognition component of the N-end rule pathway. These results indicate that some of the UBC2 functions, which include DNA repair, induced mutagenesis, sporulation, and regulation of retrotransposition, are mediated by protein degradation via the N-end rule pathway.