Effects of geographic origin on captive Macaca mulatta mitochondrial DNA variation.
Effects of geographic origin on captive Macaca mulatta mitochondrial DNA variation.
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DOI：
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发表时间：
2004-04
影响因子：
0.8
通讯作者：
S. Kanthaswamy;D. G. Smith
中科院分区：
文献类型：
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作者：
S. Kanthaswamy;D. G. Smith
从线粒体（mt）12S和16S rRNA基因的部分序列进行了分析，以表征圈养恒河猴（Macaca mulatta）来自不同地理区域的多样性。几个嵌套的分支，定义密切相关的单倍型，进行了鉴定，这表明相当大的遗传细分，可能是文物从异质起源，创始人的影响，遗传漂变，其次是育种隔离。来自印度的恒河猴母系不同离散和显着从中国母系，约90%的印度和中国恒河猴的组合样本之间的遗传异质性在这里研究是由于原产国。此外，中国猕猴的mtDNA序列比印度恒河猴更多样化，这一结果与中国更大的亚种多样性和核基因型分布一致。另外，中国恒河猴，特别是印度恒河猴的mtDNA变异的分布，表现出更少的结构，不符合一个简单的隔离距离模型。随着对遗传异质性和良好特征的恒河猴的生物医学研究的需求增加，mtDNA单倍型可以用于遗传定义，保持最大水平的遗传多样性，并确认圈养繁殖群体的恒河猴的地理起源。
Partial sequences from mitochondrial (mt) 12S and 16S rRNA genes were analyzed to characterize diversity among captive rhesus macaques (Macaca mulatta) originating from various geographic regions. Several nested clades, defined by closely related haplotypes, were identified, suggesting considerable genetic subdivision, probably relics from heterogeneous origins, founder effects, and genetic drift, followed by breeding isolation. The rhesus matrilineages from India differed discretely and markedly from Chinese matrilineages; approximately 90% of the genetic heterogeneity among the combined samples of Indian and Chinese rhesus macaques studied here was due to country of origin. In addition, mtDNA sequences from macaques of China were more diverse than those from rhesus macaques of India, an outcome consistent with China's greater subspecies diversity and with nuclear genotype distributions. Otherwise, the distribution of mtDNA variation within rhesus macaques of China, and especially within those of India, exhibited far less structure and did not conform to a simple isolation-by-distance model. As the demand for genetically heterogeneous and well-characterized rhesus macaques for biomedical-based research increases, mtDNA haplotypes can be useful for genetically defining, preserving maximal levels of genetic diversity within, and confirming the geographic origin of captive breeding groups of rhesus macaques.