Circulating complement breakdown products in patients with rheumatoid arthritis. Correlation between plasma C3d, circulating immune complexes, and clinical activity.
Circulating complement breakdown products in patients with rheumatoid arthritis. Correlation between plasma C3d, circulating immune complexes, and clinical activity.
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类风湿性关节炎患者的循环补体分解产物。
DOI：
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发表时间：
1977
影响因子：
15.9
通讯作者：
P. Miescher
中科院分区：
文献类型：
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作者：
U. Nydegger;R. Zubler;R. Gabay;G. Joliat;C. Karagevrekis;P. Lambert;P. Miescher
本文应用补体C3分解产物C3 d的定量测定方法，对30例类风湿关节炎（RA）患者的45份血浆标本进行了补体激活的研究。与退行性关节疾病患者（0.9 +/- 0.4 mg/100 ml）和健康献血者（0.8 +/- 0.5 mg/100 ml）相比，这些样本中的平均血浆C3 e水平（3.0 +/- 1.3 mg/100 ml）显著升高（P <0.001）。在79%的RA样本中，C3 d水平增加超过SD。血浆C3 d水平与滑液中的C3 d浓度进行比较。在大多数RA患者中，滑液中的C3 d水平高于血浆。观察到血浆C3 d水平与循环免疫复合物之间的非常显著的相关性，如通过测定Clq结合活性（Clq BA）所测量的（P小于0.001）。与单纯关节疾病患者（2.2 +/-1.0 mg/100 ml）相比，伴有关节外疾病表现的RA患者（3.8 +/-1.2 mg/100 ml）的C3 d水平更高。C3 d水平和Clq BA也与RA疾病活动性显著相关（P <0.001），RA疾病活动性由沉降率、关节评分和晨僵持续时间的指数表示。C3 d水平、Clq BA和临床活性之间的密切关系在随访研究中进一步显现。目前的观察结果表明，一个平行的，但相当独立的激活补体系统可能会引起免疫复合物在循环血液和关节间隙的过程中类风湿性关节炎。
Quantitative determination of the small C3 breakdown product, C3d, was used to investigate complement activation in 45 plasma samples from 30 patients with rheumatoid arthritis (RA). The mean plasma C3e level in these samples (3.0 +/- 1.3 mg/100 ml) was significantly increased (P less than 0.001) as compared to patients with degenerative joint disease (0.9 +/- 0.4 mg/100 ml) and healthy blood donors (0.8 +/- 0.5 mg/100 ml). C3d levels were increased by more than s SD in 79% of RA samples. Plasma C3d levels were compared with C3d concentrations in synovial fluid. In most RA patients, the C3d levels were higher in synovial fluid than in plasma. A very significant correlation between plasma C3d levels and circulating immune complexes, as measured by determination of Clq binding activity (Clq BA), was observed (P less than 0.001). C3d levels were more elevated in RA patients with extra-articular disease manifestations (3.8 +/- 1.2 mg/100 ml) as compared to patients with joint disease alone (2.2 +/- 1.0 mg/100 ml). C3d levels and Clq BA were also significantly correlated (P less than 0.001) with the RA disease activity expressed by an index derived from sedimentation rate, joint score, and duration of morning stiffness. A close relationship between C3d levels, Clq BA, and the clinical activity further appeared during follow-up studies. The present observations suggest that a parallel but rather independent activation of the complement system may be induced by immune complexes in circulating blood and in the joint spaces during the course of rheumatoid arthritis.