REST - A MAMMALIAN SILENCER PROTEIN THAT RESTRICTS SODIUM-CHANNEL GENE-EXPRESSION TO NEURONS
REST - A MAMMALIAN SILENCER PROTEIN THAT RESTRICTS SODIUM-CHANNEL GENE-EXPRESSION TO NEURONS
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DOI：
10.1016/0092-8674(95)90298-8
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发表时间：
1995-03-24
期刊：
影响因子：
64.5
通讯作者：
MANDEL, G
中科院分区：
文献类型：
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作者：
CHONG, JHA;TAPIARAMIREZ, J;MANDEL, G
II型电压依赖性钠通道基因的表达仅限于神经元中的神经元，这是活跃在非神经元细胞中的。我们已经克隆了与该消音器元件结合的转录因子（REST）的cDNA编码。重组休息蛋白的表达赋予了缺乏天然休息蛋白的神经元细胞类型中II型报告基因的能力，而非神经元细胞中静止的主要负面形式的表达可缓解由天然蛋白介导的沉默。在神经系统之外，会发现发育中的小鼠胚胎中的REST转录本。我们提出，神经元中II型钠通道基因的表达反映了一种默认途径，该途径通过静止的存在在非神经元细胞中阻塞。
Expression of the type II voltage-dependent sodium channel gene is restricted to neurons by a silencer element active in nonneuronal cells. We have cloned cDNA coding for a transcription factor (REST) that binds to this silencer element. Expression of a recombinant REST protein confers the ability to silence type II reporter genes in neuronal cell types lacking the native REST protein, whereas expression of a dominant negative form of REST in nonneuronal cells relieves silencing mediated by the native protein. REST transcripts in developing mouse embryos are detected ubiquitously outside of the nervous system. We propose that expression of the type II sodium channel gene in neurons reflects a default pathway that is blocked in nonneuronal cells by the presence of REST.