Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila.
Molecular Genetic Techniques for the Proteoglycan Functions in Drosophila.
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DOI：
10.1007/978-1-0716-1398-6_32
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Nakato H
中科院分区：
文献类型：
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作者：
Bowden N;Takemura M;Nakato H
几类硫酸乙酰肝素蛋白多糖(HSPG)核心蛋白和所有HS生物合成/修饰酶在进化上从人到果蝇都是保守的。这个遗传上易于处理的模型提供了高度复杂的技术，以空间和时间受控的方式操纵基因功能。因此，果蝇遗传学已经成为探索HSPGs在体内功能的强大系统。在本章中，我们将介绍果蝇可用的三种遗传技术：靶向(时间和区域基因表达靶向)、MARCM(带有可抑制细胞标记的镶嵌分析)和FLP-OUT。
Several classes of heparan sulfate proteoglycan (HSPG) core proteins and all HS biosynthetic/modifying enzymes are evolutionarily conserved from human to Drosophila melanogaster. This genetically tractable model offers highly sophisticated techniques to manipulate gene function in a spatially and temporally controlled manner. Thus, Drosophila genetics has been a powerful system to explore functions of HSPGs in vivo. In this chapter, we will introduce three genetic techniques available in Drosophila: TARGET (temporal and regional gene expression targeting), MARCM (mosaic analysis with a repressible cell marker), and FLP-Out.