Heterologous Expression of PoplarWRKY18/35Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways
Heterologous Expression of PoplarWRKY18/35Paralogs in Arabidopsis Reveals Their Antagonistic Regulation on Pathogen Resistance and Abiotic Stress Tolerance via Variable Hormonal Pathways
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杨树WRKY18/35旁系同源物在拟南芥中的异源表达揭示了它们通过可变激素途径对病原体抗性和非生物胁迫耐受性的拮抗调节
DOI：
10.3390/ijms21155440
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发表时间：
2020-08-01
影响因子：
5.6
通讯作者：
Xu, Changzheng
中科院分区：
文献类型：
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作者：
Guo, Li;Li, Chaofeng;Xu, Changzheng
WRKY转录因子（WRKY TF）是植物中最大的蛋白质家族之一，大多数通过调节相关信号通路在植物响应生物和非生物胁迫中发挥重要作用。在本研究中，我们从胡杨中分离了两个WRKY TF基因PtrWRKY 18和PtrWRKY 35，并在拟南芥中过量表达。表达谱分析表明，PtrWRKY 18和PtrWRKY 35分别对水杨酸（SA）、茉莉酸甲酯（MeJA）、脱落酸（阿坝）、B. cinereal和丁香假单胞菌治疗。转基因植株具有较高的B。实时荧光定量PCR（qRT）分析表明，PR 3和PDF 1. 2在转基因植株中表达量较高，这与它们对B的耐受性一致。灰色的转基因植物显示出比WT植物更低的烟草赤星病菌耐受性，并且qRT-PCR分析（PR 1、PR 2和NPR 1）也对应于该表型。发芽率和根系分析表明，转基因植株对阿坝的敏感性降低，导致对渗透胁迫的耐受性降低，死亡率和气孔开度增加。与野生型植株相比，一系列ABA相关基因（RD 29 A、ABO 3、ABI 4、ABI 5和DREB 1A）在PtrWRKY 18和PtrWRKY 35过表达植株中表达显著下调。这些结果表明，这两个WRKY转录因子是植物抗病性的多功能转录因子。
WRKY transcription factors (WRKY TFs) are one of the largest protein families in plants, and most of them play vital roles in response to biotic and abiotic stresses by regulating related signaling pathways. In this study, we isolated two WRKY TF genes PtrWRKY18 and PtrWRKY35 from Populus trichocarpa and overexpressed them in Arabidopsis. Expression pattern analyses showed that PtrWRKY18 and PtrWRKY35 respond to salicylic acid (SA), methyl JA (MeJA), abscisic acid (ABA), B. cinereal, and P. syringae treatment. The transgenic plants conferred higher B. cinerea tolerance than wild-type (WT) plants, and real-time quantitative (qRT)-PCR assays showed that PR3 and PDF1.2 had higher expression levels in transgenic plants, which was consistent with their tolerance to B. cinereal. The transgenic plants showed lower P. syringae tolerance than WT plants, and qRT-PCR analysis (PR1, PR2, and NPR1) also corresponded to this phenotype. Germination rate and root analysis showed that the transgenic plants are less sensitive to ABA, which leads to the reduced tolerance to osmotic stress and the increase of the death ratio and stomatal aperture. Compared with WT plants, a series of ABA-related genes (RD29A, ABO3, ABI4, ABI5, and DREB1A) were significantly down-regulated in PtrWRKY18 and PtrWRKY35 overexpression plants. All of these results demonstrated that the two WRKY TFs are multifunctional transcription factors in plant resistance.