Regional glucose metabolism and glutamatergic neurotransmission in rat brain in vivo
Regional glucose metabolism and glutamatergic neurotransmission in rat brain in vivo
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DOI：
10.1073/pnas.0405065101
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发表时间：
2004-08-24
影响因子：
11.1
通讯作者：
Behar, KL
中科院分区：
文献类型：
--
作者：
de Graaf, RA;Mason, GF;Behar, KL
在7 T下，用多波长H-1-[C-13] NMR波谱结合静脉注射[1，6-C-13（2）]葡萄糖来检测卤代乙烷麻醉大鼠脑中局部葡萄糖代谢和多巴胺能神经传递。采用基于定量T松弛映射的组织分割方法，将区域信息分解为纯大脑灰质、白色物质和皮层下结构。[4-C-13]谷氨酸、[4-C-13]谷氨酰胺和[3-C-13]谷氨酸+谷氨酰胺的13 C周转曲线与二室神经元-星形胶质细胞代谢模型拟合。大脑灰质、白色物质和皮质下的神经元三羧酸循环通量分别为0.79 +/- 0.15、0.20 +/- 0.11和0.42 +/- 0.09 mumol/min/g。大脑灰质、白色物质和皮质下的谷氨酸-谷氨酰胺神经递质循环通量分别为0.31 +/- 0.07、0.02 +/- 0.04和0.18 +/- 0.12 mumol/min/g。线粒体和胞质代谢物池之间的交换速率相对于所有脑组织类型的神经元三羧酸循环通量是快的。
Multivolume H-1-[C-13] NMR spectroscopy in combination with i.v. [1,6-C-13(2)]glucose infusion was used to detect regional glucose metabolism and glutamatergic neurotransmission in the halo-thane-anesthetized rat brain at 7 T. The regional information was decomposed into pure cerebral gray matter, white matter, and subcortical structures by means of tissue segmentation based on quantitative T, relaxation mapping. The 13C turnover curves of [4-C-13]glutamate, [4-C-13]glutamine, and [3-C-13]glutamate + glutamine were fitted with a two-compartment neuronal-astroglial metabolic model. The neuronal tricarboxylic acid cycle fluxes in cerebral gray matter, white matter, and subcortex were 0.79 +/- 0.15, 0.20 +/- 0.11, and 0.42 +/- 0.09 mumol/min per g, respectively. The glutamate-glutamine neurotransmitter cycle fluxes in cerebral gray matter, white matter, and subcortex were 0.31 +/- 0.07, 0.02 +/- 0.04, and 0.18 +/- 0.12 mumol/min per g, respectively. The exchange rate between the mitochondrial and cytosolic metabolite pools was fast relative to the neuronal tricarboxylic acid cycle flux for all cerebral tissue types.