Luteinizing hormone and prolactin in mated female meadow voles housed in long and short day lengths.
Luteinizing hormone and prolactin in mated female meadow voles housed in long and short day lengths.
复制标题
长日照和短日照下交配的雌性草甸田鼠的黄体生成素和催乳素。
DOI：
10.1095/biolreprod51.4.725
复制
发表时间：
1994
影响因子：
3.6
通讯作者：
Lee,TM
中科院分区：
文献类型：
--
作者：
Meek,LR;Lee,TM
生育力显着不同的女性草甸田鼠（Microtus pennalicus）长期（LD; 14小时光/天）和短（SD; 10小时光/天）白天的长度。所有交配的LD雌性都排卵，75-100%产窝。相比之下，40%的SD雌性在与雄性长时间接触后才交配，不排卵，SD雌性的生育能力要低得多（30-40%的雌性总体上产生窝仔）。由于交配导致排卵所需的LH激增和维持黄体所需的催乳素（PRL）增加，我们假设SD雌性可能由于激素反应不足而具有较低的排卵率和窝产。血清LH测定前24小时，30，60，90，120和180分钟后，交配。催乳素测定前，24和48小时后，交配。LD和SD女性排卵表现出LH水平急剧上升，在交配后30和60分钟，但LH在非排卵女性约50%低于排卵女性在交配后30和60分钟。催乳素在LD女性交配后24和48小时的两倍基线水平。SD雌鼠交配后24 h PRL升高，交配后48 h下降至交配前水平，表明短日照条件下雌鼠生育力下降可能与两个不同的问题有关：1）交配后LH升高不足以引起排卵; 2)PRL可能不会保持足够长的时间来支持排卵女性的功能性黄体。
Fertility differs dramatically between female meadow voles (Microtus pennsylvanicus) housed in long (LD; 14 h light/day) and short (SD; 10 h light/day) day lengths. All mated LD females ovulate, and 75–100% produce litters. In contrast, 40% of SD females that mate only after long contact with a male do not ovulate, and fertility for SD females is much lower (30–40% produce litters overall). Because copulation causes the surge in LH required for ovulation and the increase in prolactin (PRL) needed to maintain corpora lutea, we hypothesized that SD females might have lower ovulation rates and litter production because of inadequate hormonal responses. Serum LH was measured 24 h before, and 30, 60, 90, 120, and 180 min after, mating. Prolactin was measured before, and 24 and 48 h after, copulation.LD and SD females that ovulated demonstrated a sharp increase in LH levels at 30 and 60 min post mating, but LH in nonovulating females was approximately 50% lower than that of ovulating females at 30 and 60 min post mating.PRL in LD females was double baseline levels at 24 and 48 h after mating. PRL in SD females increased at 24 h, but declined to pre-mating levels by 48 h post mating.These data suggest that reduced fertility in short day lengths in female voles may be related to two separate problems: 1) The rise in LH following copulation is insufficient in some females to cause ovulation. 2) PRL may not remain elevated long enough to support functional corpora lutea in some females that ovulate.