An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral Salmonella Infection.
An Outer Membrane Vesicle-Adjuvanted Oral Vaccine Protects Against Lethal, Oral Salmonella Infection.
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DOI：
10.3390/pathogens10050616
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发表时间：
2021-05-18
期刊：
影响因子：
--
通讯作者：
McLachlan JB
中科院分区：
文献类型：
--
作者：
Harrell JE;Kurtz JR;Bauer DL;Prior JT;Gellings PS;Morici LA;McLachlan JB
由鼠伤寒沙门氏菌引起的非伤寒沙门氏菌病是一种常见的粪口疾病，以轻度胃肠道不适为特征，导致腹泻、寒战、发热、腹部痉挛、头痛和身体疼痛、恶心和呕吐。越来越多的抗药性侵袭性非伤寒沙门氏菌感染使这一全球威胁需要新的治疗策略，包括下一代疫苗。目前这项研究的目标是研制一种可口服的新型沙门氏菌疫苗平台。为了实现这一点，我们创造了一种沙门氏菌特异性疫苗，辅以假鼻疽伯克霍尔德氏菌外膜囊泡(OMV)。我们证明，将OMVS添加到热灭活口服沙门氏菌疫苗(HKST+OMVS)中可以防止沙门氏菌的致命口服挑战。此外，我们还发现免疫后可诱导出调理抗沙门氏菌抗体，当口服佐剂使用OMV时可诱导出CD4T细胞和B细胞。这项研究代表了一种新的口服疫苗方法，以对抗日益严重的侵袭性沙门氏菌感染问题。
Non-typhoidal salmonellosis, caused by Salmonella enterica serovar Typhimurium is a common fecal-oral disease characterized by mild gastrointestinal distress resulting in diarrhea, chills, fever, abdominal cramps, head and body aches, nausea, and vomiting. Increasing incidences of antibiotic resistant invasive non-typhoidal Salmonella infections makes this a global threat requiring novel treatment strategies including next-generation vaccines. The goal of the current study was to formulate a novel vaccine platform against Salmonella infection that could be delivered orally. To accomplish this, we created a Salmonella-specific vaccine adjuvanted with Burkholderia pseudomallei outer membrane vesicles (OMVs). We show that adding OMVs to a heat-killed oral Salmonella vaccine (HKST + OMVs) protects against a lethal, oral challenge with Salmonella. Further, we show that opsonizing anti-Salmonella antibodies are induced in response to immunization and that CD4 T cells and B cells can be induced when OMVs are used as the oral adjuvant. This study represents a novel oral vaccine approach to combatting the increasing problem of invasive Salmonella infections.
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鼠伤寒沙门氏菌突变体衍生的带有截短脂多糖的外膜囊泡在小鼠模型中诱导针对肠道沙门氏菌血清型感染的交叉保护性免疫反应
DOI：
10.1016/j.ijmm.2016.08.004
发表时间：
2016-12
期刊：
International journal of medical microbiology : IJMM
影响因子：
--
作者：
Liu Q;Liu Q;Yi J;Liang K;Liu T;Roland KL;Jiang Y;Kong Q
通讯作者：
Kong Q
影响因子：
168.9
作者：
Feasey, Nicholas A.;Dougan, Gordon;Kingsley, Robert A.;Heyderman, Robert S.;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
6.4
作者：
Ferreira RB;Valdez Y;Coombes BK;Sad S;Gouw JW;Brown EM;Li Y;Grassl GA;Antunes LC;Gill N;Truong M;Scholz R;Reynolds LA;Krishnan L;Zafer AA;Sal-Man N;Lowden MJ;Auweter SD;Foster LJ;Finlay BB
通讯作者：
Finlay BB
影响因子：
29.4
作者：
MICHETTI, P;PORTA, N;NEUTRA, MR
通讯作者：
NEUTRA, MR
影响因子：
3.8
作者：
CARLIN, NIA;SVENSON, SB;LINDBERG, AA
通讯作者：
LINDBERG, AA