Foxp3+Helios+ regulatory T cells are associated with monocyte subsets and their PD-1 expression during acute HIV-1 infection
Foxp3+Helios+ regulatory T cells are associated with monocyte subsets and their PD-1 expression during acute HIV-1 infection
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Foxp3( )Helios( ) 调节性 T 细胞与急性 HIV-1 感染期间的单核细胞亚群及其 PD-1 表达相关
DOI：
10.1186/s12865-019-0319-7
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发表时间：
2019-10-24
期刊：
影响因子：
3
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Liu, Lifeng;Zhang, Qiuyue;Zhang, Tong
背景Helios已被报道稳定调节性T（Treg）抑制功能。三种人类单核细胞亚群中程序性细胞死亡蛋白1（PD-1）的表达调节免疫反应。最近，我们的研究小组报道了三种单核细胞亚群与HIV-1感染患者的T辅助细胞分化有关。到目前为止，单核细胞亚群及其PD-1表达对Foxp 3（+）Helios（+）Treg细胞的影响尚未完全表征，特别是在急性HIV-1感染期间。结果急性HIV-1感染者Foxp 3（+）Helios（+）CD 45 RA（+）Treg细胞的频率显著高于健康对照组和接受联合抗逆转录病毒治疗的慢性HIV-1感染者。Foxp 3（+）Helios（+）CD 45 RA（+）Treg细胞的频率与慢性HIV-1感染患者的CD 4 T细胞计数和CD 4/CD 8比值呈负相关。在急性HIV-1感染期间，Foxp 3（+）Helios（+）CD 45 RA（+）Treg细胞的频率与中间CD 14（++）CD 16（+）单核细胞亚群的频率呈负相关，但与中间CD 14（++）CD 16（+）和非经典CD 14（+）CD 16（++）单核细胞亚群中的PD-1表达呈正相关。结论HIV-1感染过程中，Foxp 3（+）Helios（+）Treg细胞的变化是HIV-1感染过程中单核细胞亚群及其PD-1表达与Foxp 3（+）Helios（+）Treg细胞的关系。我们的观察为Foxp 3（+）Helios（+）Treg细胞和单核细胞亚群上的PD-1表达在HIV发病机制中的作用提供了新的证据。
Background Helios has been reported to stabilize regulatory T (Treg) suppressive function. Programmed cell death protein 1 (PD-1) expression in three human monocyte subsets modulates immune responses. Recently, our team reported that three monocyte subsets are associated with T helper cell differentiation in HIV-1-infected patients. Until now, the effects of monocyte subsets and their PD-1 expression on Foxp3(+)Helios(+) Treg cells have not been fully characterized, especially during acute HIV-1 infection. Results The frequency of Foxp3(+)Helios(+)CD45RA(+) Treg cells is significantly higher in patients with acute HIV-1 infection than those of healthy controls and chronic HIV-1-infected patients undergoing combined antiretroviral therapy. The frequency of Foxp3(+)Helios(+)CD45RA(+) Treg cells is inversely correlated with CD4 T-cell counts and the CD4/CD8 ratio in chronic HIV-1-infected patients. During acute HIV-1 infection, the frequency of Foxp3(+)Helios(+)CD45RA(+) Treg cells is inversely correlated with the frequency of the intermediate CD14(++)CD16(+) monocyte subset, but positively correlated with PD-1 expression in both intermediate CD14(++)CD16(+) and non-classical CD14(+)CD16(++) monocyte subsets. Conclusions In this study, the perturbations of Foxp3(+)Helios(+) Treg cells were characterized, and the association between monocyte subsets and their PD-1 expression and Foxp3(+)Helios(+) Treg cells was evaluated during HIV-1 infection. Our observations provide new evidence of the roles for Foxp3(+)Helios(+) Treg cells and PD-1 expression on monocyte subsets in HIV pathogenesis.