Comparison of C-reactive protein levels between serum and plasma samples on long-term frozen storage after a 13.8 year interval: The JMS cohort study
Comparison of C-reactive protein levels between serum and plasma samples on long-term frozen storage after a 13.8 year interval: The JMS cohort study
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DOI：
10.2188/jea.17.120
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发表时间：
2007-07-01
影响因子：
4.7
通讯作者：
Kajii, Eiji
中科院分区：
文献类型：
--
作者：
Ishikawa, Shizukiyo;Kayaba, Kazunori;Kajii, Eiji
背景：C反应蛋白（CRP）是一种急性时相反应物，被认为是心血管疾病的预测因子。解冻样本中高灵敏度CRP的测量是可能的，并且认为即使在冷冻储存后，该值也保持稳定。然而，CRP值的长期稳定性尚未记录。我们测量CRP的值之前和之后的长期storage.Methods：高灵敏度CRP测定之前和之后的长期存储的样本从99名男性和女性的JMS队列研究的主题，并检查的差异。我们采用分层抽样方法，使用基线CRP值，选择在基线时接受高敏CRP测量的受试者。CRP测定血清样本在基线和解冻血浆样本后，平均储存期为13.8year.Results：CRP的几何平均值为0.25 mg/L和0.59 mg/L，储存前和后，分别。长期冻存后CRP值显著高于基线（p
Background: C-reactive protein (CRP) is an acute phase reactant, and has been reported as a predictor of cardiovascular diseases. Measurements of high sensitive CRP in thawed samples are possible and the values are thought to remain stable even after frozen storage. However, the long-term stability of CRP values has not been documented. We measured the values of CRP before and after long-term storage, and examined the difference in determined values.Methods: High sensitive CRP was measured before and after long-term storage of samples from 99 men and women among the JMS Cohort Study subjects. We selected subjects who underwent measurement of high sensitive CRP at the baseline by stratified sampling methods using baseline CRP values. CRP was measured in serum samples at the baseline and in thawed plasma samples after an average storage period of 13.8 years.Results: Geometric means of CRP were 0.25 mg/L and 0.59 mg/L before and after storage, respectively. The CRP values were significantly higher after long-term frozen storage than at the baseline (p