13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine
13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine
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DOI：
10.1002/mrm.21650
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发表时间：
2008-08-01
影响因子：
3.3
通讯作者：
Brindle, Kevin M.
中科院分区：
文献类型：
--
作者：
Gallagher, Ferdia A.;Kettunen, Mikko I.;Brindle, Kevin M.
动态核极化（DNP）是一种用于提高C-13 MR波谱（MRS）灵敏度的新兴技术。[5-C-13（1）]使用该技术使谷氨酰胺超极化高达5%，代表灵敏度增加6000倍。在培养的人肝癌细胞（HepG 2）中，使用C-13-MRS测量证实了线粒体谷氨酰胺酶将超极化的谷氨酰胺转化为谷氨酸。这些结果代表了第一步，在发展中国家的成像技术，用于检测谷氨酰胺代谢在体内。此外，由于谷氨酰胺的利用与细胞增殖相关，该研究提出了一种检测肿瘤细胞增殖变化的新技术。
Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of C-13 MR spectroscopy (MRS). [5-C-13(1)]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000-fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using C-13-MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation.