Fate of prions in soil: Longevity and migration of recPrP in soil columns
Fate of prions in soil: Longevity and migration of recPrP in soil columns
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DOI：
10.1016/j.soilbio.2006.12.030
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发表时间：
2007-05-01
影响因子：
9.7
通讯作者：
Shaw, George
中科院分区：
文献类型：
--
作者：
Cooke, Cindy M.;Shaw, George
近年来，对传染性海绵状脑病(TSE)的研究已成为世界范围内的高度优先事项，但人们对TSE在环境中的传染性行为知之甚少。Pron蛋白的弹性和稳定性可能会导致土壤成为TSE感染性的潜在宿主，原因是受感染的身体埋葬或屠宰场的污水扩散等活动造成的污染，或受感染的动物对牧场的污染(例如绵羊的瘙痒病)。了解Pron蛋白质在土壤中的去向，以及有利于迁移的相关物理化学条件，可以用来帮助防止通过放牧再次感染动物，保护水道和制定良好的管理做法。在连续9个月和6个月的两个试验中，观察了不同微生物活性水平(正常和降低)、不同土壤水分和氧化还原电位以及两种不同土壤类型(壤质砂土和粘壤土)下重组绵羊PrP(RecPrP)在土柱中的迁移情况。在每个分析时间(1、3、6或9个月)，在两种土壤类型的原始污染层中都检测到全长recPrP，表明recPrP在不同土壤条件下的弹性和稳定性，即使在存在活跃的土壤微生物种群的情况下也是如此。在最早的分析时间(1或3个月)，在每个土柱中都发现了蛋白质迁移的证据，但最大距离限制在1厘米，这表明土壤中的初始移动性有限，随后在接下来的几天到几周内有很强的吸附。RecPrP在土壤中的存活期至少为9个月。在这项研究中，recPrP被用作潜在的TSE传染性的指标，尽管在对土壤中的普恩病毒构成的感染风险得出结论之前，应该进行传染性测试。然而，已经证明，土壤很可能成为污染物质在储存或埋葬地点扩散的重要障碍。(C)2007爱思唯尔有限公司。保留所有权利。
Research into transmissible spongiform encephalopathy (TSE) diseases has become a high priority worldwide in recent years yet remarkably little is known about the behaviour of TSE infectivity in the environment. The resilience and stability of prion proteins could lead to soils becoming a potential reservoir of TSE infectivity as a result of contamination from activities such as infected carcass burial or the dispersion of effluents from slaughter houses, or by contamination of pastures by infected animals, (e.g. scrapie in sheep). Knowledge of the fate of prion proteins in soils, and associated physico-chemical conditions which favour migration, can be used to help prevent re-infection of animals through grazing, to protect watercourses and develop good management practices. In two consecutive experiments of 9 and 6 months, the migration of recombinant ovine PrP (recPrP) in soil columns was followed under contrasting levels of microbial activity (normal versus reduced), under varying regimes of soil water content and redox potential, and in two different soil types (loamy sand and clay loam). At each analysis time (1, 3, 6 or 9 months), in both soil types, full-length recPrP was detected in the original contaminated layer, indicating the resilience and stability of recPrP under varied soil conditions, even in the presence of active soil microbial populations. Evidence of protein migration was found in every soil column at the earliest analysis time (1 or 3 months), but was restricted to a maximum distance of 1 cm, indicative of limited initial mobility in soils followed by strong adsorption over the following days to weeks. The survival of recPrP in the soil over a period of at least 9 months was demonstrated. In this study, recPrP was used as an indicator for potential TSE infectivity, although infectivity tests should be carried out before conclusions can be drawn regarding the infection risk posed by prions in soil. However, it has been demonstrated that soil is likely to act as a significant barrier to the dispersion of contaminated material at storage or burial sites. (c) 2007 Elsevier Ltd. All rights reserved.