Xueshuantong Injection (Lyophilized) Attenuates Cerebral Ischemia/Reperfusion Injury by the Activation of Nrf2-VEGF Pathway
Xueshuantong Injection (Lyophilized) Attenuates Cerebral Ischemia/Reperfusion Injury by the Activation of Nrf2-VEGF Pathway
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DOI：
10.1007/s11064-018-2523-x
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发表时间：
2018-05-01
影响因子：
4.4
通讯作者：
Hu, Limin
中科院分区：
文献类型：
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作者：
Guo, Hong;Adah, Dickson;Hu, Limin
血栓通注射液（冻干品，XST）是从传统中药三七中提取的，对脑缺血有神经保护作用。缺血组织的再血管化有利于脑血管病的治疗。本研究旨在探讨XST对脑缺血的促血管生成作用及其可能的作用机制。采用大鼠大脑中动脉缺血模型，连续3天或7天腹腔注射XST。评价神经功能缺损和内源性抗氧化能力。通过ELISA和免疫组化染色评估卒中后血管生成和血管化。采用实时荧光定量RT-PCR方法检测脑组织中Nrf 2、HO-1、NQO 1的转录水平。结果表明，XST能明显改善MCAO后神经元功能缺损，促进血管新生和血管化。血管生成的机制可能与内源性抗氧化能力和Nrf 2通路有关。结论：脑卒中后7 d给予XST可显著改善功能恢复，促进血管新生，其机制可能与Nrf 2信号通路有关。本研究结果为XST治疗脑缺血性疾病提供了科学依据，并为进一步研究提供了理论支持。
Xueshuantong injection (Lyophilized, XST), extracted from the traditional Chinese medicinal herb Panax notoginseng, has neuroprotective effect on cerebral ischemia. Revascularization of ischemic tissue is good for the therapy of cerebrovascular disease. In this study, angiogenic potentiality and possible mechanism of XST for cerebral ischemia were explored. Rats were subjected to transient middle cerebral artery occlusion (MCAO), and then intraperitoneally administered with XST daily for 3 or 7 consecutive days. The neurological function deficits, and endogenous antioxidant capacity were evaluated. Post-stroke angiogenesis and vascularization were assessed by ELISA and immunohistochemical staining. Transcription levels of Nrf2, HO-1, NQO1 in brain tissues were analyzed by real-time RT-PCR. The results showed that XST could remarkably ameliorate neuronal functional deficit, promote angiogenesis and vascularization after MCAO. The mechanism of angiogenesis might be related to endogenous antioxidant capacity and Nrf2 pathway. In conclusion, administered XST for 7 days after stroke could significantly improve functional recovery and promote angiogenesis, that might be related to Nrf2 signaling pathway. These findings could provide scientific evidence for the use of XST in cerebral ischemic diseases and provide theoretical support for further studies.